A decoy receptor derived from alternative splicing fine-tunes cytokinin signaling in Arabidopsis  

作　　者：Michaela Králová Ivona Kubalová Jakub Hajný Karolina Kubiasová Karolína Vagaská Zengxiang Ge Michelle Gallei Hana Semerádová Anna Kuchařová Martin Hönig Aline Monzer Martin Kovačik JiříFriml Ondřej Novák Eva Benková Yoshihisa Ikeda David Zalabák 

机构地区：[1]Laboratory of Growth Regulators,Faculty of Science of PalackýUniversity and Institute of Experimental Botany AS CR,Šlechtitelů27,78371 Olomouc,Czech Republic [2]Department of Molecular Biology,Centre of the Region Hanáfor Biotechnological and Agricultural Research,Faculty of Science,PalackýUniversity,Šlechtitelů27,78371 Olomouc,Czech Republic [3]Institute of Science and Technology Austria(ISTA),3400 Klosterneuburg,Austria [4]Department of Chemical Biology,Faculty of Science,PalackýUniversity,Šlechtitelů27,78371 Olomouc,Czech Republic [5]Institute of Experimental Botany of the Czech Academy of Sciences,Centre of Plant Structural and Functional Genomics,Šlechtitelů31,77900 Olomouc,Czech Republic [6]These authors contributed equally to this article.

出　　处：《Molecular Plant》2024年第12期1850-1865,共16页分子植物（英文版）

基　　金：Czech Science Foundation via project 23-07363S(to D.Z.,J.H.,K.V.,and O.N.);Scientific Service Units(SSU)of ISTA through resources provided by the Imaging and Optics Facility and the Life Science Facility.

摘　　要：Hormone perception and signaling pathways have a fundamental regulatory function in the physiological processes of plants.Cytokinins,a class of plant hormones,regulate cell division and meristem maintenance.The cytokinin signaling pathway is well established in the model plant Arabidopsisthaliana.Several negative feedback mechanisms,tightly controlling cytokinin signaling output,have been described previously.In this study,we identified a new feedback mechanism executed through alternative splicing of the cytokinin receptor AHK4/CRE1.A novel splicing variant named CRE1int7 results from seventh intron retention,introducing a premature termination codon in the transcript.We showed that CRE1int7 is translated in planta into a truncated receptor lacking the C-terminal receiver domain essential for signal transduction.CRE1int7 can bind cytokinin but cannot activate the downstream cascade.We present a novel negative feedback mechanism of the cytokinin signaling pathway,facilitated by a decoy receptor that can inactivate canonical cytokinin receptors via dimerization and compete with them for ligand binding.Ensuring proper plant growth and development requires precise control of the cytokinin signaling pathway at several levels.CRE1int7 represents a so-far unknown mechanism for fine-tuning the cytokinin signaling pathway in Arabidopsis.

关 键 词：SPLICING ARABIDOPSIS DECOY 

分 类 号：Q94[生物学—植物学]