检索规则说明:AND代表“并且”;OR代表“或者”;NOT代表“不包含”;(注意必须大写,运算符两边需空一格)
检 索 范 例 :范例一: (K=图书馆学 OR K=情报学) AND A=范并思 范例二:J=计算机应用与软件 AND (U=C++ OR U=Basic) NOT M=Visual
作 者:侯田田 秦超 王涛 王月丹[3] 张秀军 姚志伟 黄瑛[1] 耿兴超[1] HOU Tiantian;QIN Chao;WANG Tao;WANG Yuedan;ZHANG Xiujun;YAO Zhiwei;HUANG Ying;GENG Xingchao(Beijing Key Laboratory for Safety Evaluation of Drugs,National Center for Safety Evaluation of Drugs,National Institutes for Food and Drug Control,Beijing 100176,China;The Fifth Medical Center of the PLA General Hospital,Beijing 100039,China;Key Laboratory of Medical Immunology,National Health Commission,School of Basic Medical Sciences Peking University,Beijing 100191,China;Aeonvital Institute of Clinical and Translational Immunology,Beijing 102600,China;SanQ(Beijing)Biotechnology Co.,Ltd.,Beijing 100176,China)
机构地区:[1]中国食品药品检定研究院国家药物安全评价监测中心,药物非临床安全评价研究北京市重点实验室,北京100176 [2]解放军总医院第五医学中心,北京100039 [3]北京大学基础医学院,国家卫生健康委员会医学免疫学重点实验室,北京100191 [4]北京循生免疫医学转化研究院,北京102600 [5]三卿(北京)生物科技有限公司,北京100176
出 处:《中国药学杂志》2024年第20期1910-1916,共7页Chinese Pharmaceutical Journal
基 金:中国食品药品检定研究院中青年发展研究基金资助(2024C6);国家重点研发计划课题资助(2021YFA1101602)。
摘 要:目的 评估肿瘤浸润淋巴细胞(tumor infiltrating lymphocyte, TIL)在NOG小鼠体内的分布及清除情况。方法 研究共使用84只NOG小鼠,分为溶媒对照组和受试物组,单次尾静脉注射给予小鼠TIL,在给药后不同时间点采血和解剖,取脏器,采用流式细胞术和实时定量聚合酶链反应(real-time quantitative PCR,qPCR)方法检测细胞在血液和不同脏器的分布情况。结果 给药后1~2 d,血液中CD3^(+)、CD3^(+)CD4^(+)、CD3^(+)CD8^(+)细胞数量达到峰值,同时qPCR检测结果显示,TIL主要分布于肺、血液、肝脏等组织,其他组织分布较少,给药后42 d,大部分组织基因拷贝低于定量下限。结论 NOG小鼠单次尾静脉注射TIL,主要在肺脏、血液、肝脏中分布,并且在体内存续时间不超过42 d。OBJECTIVE To evaluate the distribution and clearance of tumor infiltrating lymphocytes(TIL)in NOG mice.METHODS Eighty-four NOG mice were used,divided into control group and test group.The mice were given a single tail vein injection.After administration blood was collected and organs were taken at different time points,flow cytometry and real-time quantitative PCR(qPCR)were used to detect the distribution of cells in the blood and different organs.RESULTS After 1-2 d of administration,the number of CD3^(+),CD3^(+)CD4^(+),and CD3^(+)CD8^(+)cells in the blood reached their peaks.At the same time,qPCR showed that TIL was mainly distributed in tissues such as lungs,blood,and liver,with less distribution in other tissues.On day 42 after administration,gene copies of most tissues were below the lower limit of quantitation.CONCLUSION Single tail vein injection of TIL in NOG mice is mainly distributed in the lungs,blood,and liver,and its duration in vivo does not exceed 42 d.
关 键 词:肿瘤浸润淋巴细胞 生物分布 流式细胞术 实时定量聚合酶链反应
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在链接到云南高校图书馆文献保障联盟下载...
云南高校图书馆联盟文献共享服务平台 版权所有©
您的IP:216.73.216.198