鲜用艾叶萜类成分及其抗炎活性研究  

Terpenoid Constituents from the Fresh-Used Leaves of Artemisia argyi and Their Anti-Inflammatory Activities

作　　者：李齐激 李雨铭 王丽 周浪 陈发菊 杨小生 LI Qiji;LI Yuming;WANG Li;ZHOU Lang;CHEN Faju;YANG Xiaosheng(Engineering Research Center for Efficient Utilization of Natural Products in Cuizhou Province,Natural Products Research Center of Guizhou Province,Guiyang 550014,China;State Key Laboratory of Functions and Applications of Medicinal Plants,Guizhou Medical University,Guiyang 550025,China)

机构地区：[1]贵州省天然产物研究中心,贵州省天然产物高效利用工程技术研究中心,贵阳550014 [2]贵州医科大学,省部共建药用植物功效与利用国家重点实验室,贵阳550025

出　　处：《中国药学杂志》2024年第19期1807-1812,共6页Chinese Pharmaceutical Journal

基　　金：贵州省科技创新能力建设专项资助(黔科合服企[2020]4013号);贵州省天然产物高效利用工程研究中心项目资助(黔财建[2019]303);贵州食药用生物资源可持续利用特色重点实验室项目资助(黔教合KY字[2020]018)。

摘　　要：目的研究新鲜艾叶(Artemisia argyi Levi.et Vant.)中萜类成分及其抗炎效果。方法利用高效制备液相色谱等现代植物化学分离技术对新鲜艾叶体积分数95%乙醇提取物进行分离,结合理化性质与质谱法(MS)、核磁共振谱法(NMR)等数据鉴定结构。以脂多糖(LPS)诱导RAW264.7巨噬细胞释放一氧化氮(NO)抑制能力评价萜类物质的抗炎能力。结果共分离15个萜类物质:artanomaloide(1)、dehydroleucodin(2)、tamaulipin-B acetate(3)、莫沙坦内酯(4)、yomogin(5)、木香酸(6)、costic acid methyl ester(7)、PBI(8)、(-)-3-hydroxy-β-ionone(9)、pubinernoid A(10)、subamone(11)、artefrigin(12)、cassipourol(13)、3β-acetoxy-24-oxo-dammara-20,25-diene(14)、dammara-20,24-dien-3β-ol acetate(15),其中化合物1~12抗炎活性IC50值介于(1.27±0.02)~(17.55±0.13)μmol·L^(-1)。结论鲜用艾叶中含有较为丰富的、具有明显抗炎活性的萜类物质,其中化合物3、7~14均为首次从艾叶中分离,该类成分有进一步在医药大健康产品中开发应用的潜能。OBJECTIVE To investigate the terpenoid constituents from the fresh-used leaves of Artemisia argyi Levl.et Vant.and their anti-inflammatory activities.METHODS Modern phytochemical separation techniques such as high performance preparative liquid chromatography(HPLC)were used to isolate the 95%ethanol extract of the fresh leaves from A.argyi and to identify the structure by combining physicochemical properties with MS and NMR data.The anti-inflammatory activity of terpenoids was evaluated by the inhibitory ability of nitric oxide(NO)release from LPS-induced RAW264.7 macrophages.RESULTS Fifteen terpenoids were isolated from the fresh leaves of A.argyi and identified as artanomaloide(1),dehydroleucodin(2),tamaulipin-B acetate(3),moxartenolide(4),yomogin(5),costic acid(6),costic acid methyl ester(7),PBI(8),(-)-3-hydroxy-β-ionone(9),pubinernoid A(10),subamone(11),artefrigin(12),cassipourol(13),3β-acetoxy-24-oxo-dammara-20,25-diene(14),dammara-20,24-dien-3β-ol acetate(15).The IC50 values of the anti-inflammatory activity of compounds 1-12 ranged from(1.27±0.02)to(17.55±0.13)μmol·L^(-1).CONCLUSION The fresh leaves from A.argyi are more abundant in terpenoids with anti-inflammatory activity,among which compounds 3 and 7-14 are isolated for the first time,and this kind of components can be applied in the development of pharmaceutical and health products.

关 键 词：艾叶 鲜用 萜类成分 抗炎活性 

分 类 号：R282[医药卫生—中药学]