基于植物代谢组学方法的陕产黄精不同炮制品化学差异研究  

Analysis of Chemical Variability on Different Processed Products of Polygonati Rhizoma Produced in Shaanxi Province Based on Plant Metabolomics

作　　者：宋艺君[1,2] 孙婧 郭涛[3] 高阳丽 宋瑞 卢旭阳 张凯 张桥 赵重博 孙静[1,2] SONG Yijun;SUN Jing;GUO Tao;GAO Yangli;SONG Rui;LU Xuyang;ZHANG Kai;ZHANG Qiao;ZHAO Chongbo;SUN Jing(College of Pharmacy,Shaanxi University of Chinese Medicine,Xianyang 712046,China;Shaanxi Engineering Technology Research Center of Prepared Slices,Xianyang 712046,China;No 986 Hospital of PLAAF,Xi'an 710054,China)

机构地区：[1]陕西中医药大学药学院,陕西咸阳712046 [2]陕西省中药饮片工程技术研究中心,陕西咸阳712046 [3]空军第九八六医院,西安710054

出　　处：《中国药学杂志》2024年第21期2011-2021,共11页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金项目资助(82104395);陕西省科技厅项目资助(2024SF-YBXM-456);陕西省高水平中医药重点学科资助(中药炮制学)(174010324027/2024)。

摘　　要：目的分析陕产黄精不同炮制品化学成分的差异。方法采用超高效液相色谱-静电场轨道阱高分辨质谱(UPLC-Q-Exactive Orbitrap-MS)技术对陕产黄精不同炮制品的化学成分进行测定,所得数据采用主成分分析(PCA)法与正交偏最小二乘判别分析(OPLS-DA)处理,陕产黄精不同炮制品的差异指标成分通过精准的一级质谱质荷比和二级质谱碎片离子同对照品图谱和软件数据库搜索以及相关文献报道成分进行判别分析。结果共鉴定得到45个成分,通过OPLS-DA,筛选出不同炮制品之间差异性化学成分23个,包括氨基酸类13个,生物碱类5个,糖类2个,香豆素类1个,皂苷类1个,有机酸类1个。结论黄精、蒸黄精、酒黄精存在一定的化学成分差异,可为黄精炮制品的质量控制和药效研究奠定物质基础。OBJECTIVE To analyze the difference of chemical components of different processed products of Polygonati Rhizoma produced in Shaanxi Province.METHODS Ultra-high performance liquid chromatography coupled with hybrid quadrupole-orbitrap mass spectrometry(UPLC-Q-Exactive Orbitrap-MS)was developed to determine the chemical components of different processed products of Polygonati Rhizoma produced in Shaanxi Province.The data was analyzed by principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA).The structures of chemical markers of different processed products of the plants were identified based on accurate primary mass spectrometry and secondary mass spectrometry fragment ion,combined with the reference map,software database searching and related literature.RESULTS A total of 45 components were identified,there were 23 chemical components with significant differences distinguished by the method of OPLS-DA,including 13 amino acids,5 alkaloids,2 saccharides,1 coumarin,1 saponin,1 organic acids.CONCLUSION There are some chemical differences among the three products.This study can provide material basis for the quality control and pharmacodynamic research of processed products of Polygonati Rhizoma.

关 键 词：黄精 蒸黄精 酒黄精 不同炮制品 化学差异 植物代谢组学 

分 类 号：R284[医药卫生—中药学]