Solvent-free synthesis of diacylglycerols via enzymatic glycerolysis between edible oils and glycerol catalyzed by self-made immobilized lipase PS@LXTE-1000  

作　　者：Yuhao Li Yi Zhang Run Liu Zhonghui Liu Kheng-Lim Goh Vladimir Zivkovic Mingming Zheng 

机构地区：[1]School of Chemical and Environmental Engineering,Wuhan Polytechnic University,Wuhan,Hubei,430023,China [2]Oil Crops Research Institute,Chinese Academy of Agricultural Sciences,Hubei Key Laboratory of Lipid Chemistry and Nutrition,Hubei Hongshan Laboratory,Key Laboratory of Oilseeds Processing,Ministry of Agriculture,Wuhan,430062,China [3]Newcastle University in Singapore,172A Ang Mo Kio Avenue 8#05-01,599493,Singapore [4]School of Engineering,Newcastle University,Newcastle Upon Tyne,NE17RU,UK

出　　处：《Oil Crop Science》2024年第4期225-233,共9页中国油料作物学报(英文版）

基　　金：supported by the National Key Research and Development Project of China(2021YFD2100303);the National Natural Science Foundation of China(32272271,32302021);the Hubei Province Natural Science Foundation of China(2021CFB209,2023AFB324);the Major Project of Hubei Hongshan Laboratory(2022hszd002);Central Public-interest Scientific Institution Basal Research Fund(No.1610172024002).

摘　　要：Diglycerol(DAG)is a structural lipid with the functions to lower body fat accumulation and decrease serum triglyceride level.However,the enzymatic synthesis of DAG is limited by the high-efficient and economic lipases.In this paper,the immobilized lipase PS@LXTE-1000 was self-made by immobilizing the Pseudomomas cepacian lipase on to the hydrophobic microporous resin LXTE-1000.The results indicate that LXTE-1000 was a uniform mesoporous sphere with the mean diameter of 400μm,pore size of 14.6 nm,pore volume of 0.5 cm3/g and surface area of 126.0 m^(2)/g,showing superior structural properties for lipase immobilization.Under the optimal reaction conditions with the molar ratio of rapeseed oil to glycerol being 1:1,adding amount of immobilized lipase being 4%,reaction at 50℃,the highest DAG content of 46.7%was achieved in 3 h via enzymatic glycerolysis catalyzed by LXTE-1000.After 7 cycles of reuse,the self-made LXTE-1000 could still retain 78.3%of its initial catalytic ability.Besides,LXTE-1000 was observed to facilitate the DAG production via glycerolysis reaction between glycerol with other seven edible oils including corn oil,sesame oil,peony seed oil,rice bran oil,peanut oil,soybean oil and flaxseed oil.Specifically,the glycerolysis reaction with sesame oil,peony seed oil and rice bran oil even led to the DAG content of 52.1%,53.3%and 51.2%,respectively,Hence,this paper provide a novel strategy to produce high-efficient and economic immobilized lipases,which shows great potential in the green synthesis of functional lipids such as DAG.

关 键 词：Immobilized lipase Diaglycerol Enzymatic glycerolysis Functional lipids 

分 类 号：TS221[轻工技术与工程—粮食、油脂及植物蛋白工程]