双歧杆菌R0033和R0071联合乳糖酶干预乳糖不耐受婴儿效果及对肠道菌群的影响:一项随机对照试验  

Effect of bifidobacterium R0033 and R0071 combined with lactase intervention on lactose intolerance in infants and gut microbiota:a randomized controlled study

作　　者：周锋 宋琪 孙海岚 ZHOU Feng;SONG Qi;SUN Hai Lan(Chongqing Health Center for Women and Children(Women and Children’s Hospital of Chongqing Medical University),Chongqing 401147,China)

机构地区：[1]重庆市妇幼保健院(重庆医科大学附属妇女儿童医院),重庆401147

出　　处：《中国妇幼卫生杂志》2024年第6期39-44,共6页Chinese Journal of Women and Children Health

基　　金：中国疾病预防控制中心妇幼保健中心2022年度“母婴营养与健康研究项目”(2022FYH003)。

摘　　要：目的 探讨双歧杆菌R0033和R0071联合乳糖酶干预婴儿乳糖不耐受引起的慢性腹泻的临床效果,以便为临床治疗提供新的思路和参考。方法 选取2023年3-11月重庆市妇幼保健院收治的34例乳糖不耐受患儿作为研究对象,通过随机数字表法分为对照组和观察组,每组各17例。对照组仅采用乳糖酶干预,观察组在乳糖酶干预基础上,加用婴儿双歧杆菌R0033和两歧双歧杆菌R0071。比较两组患儿一般资料和干预1 W前后排便次数、腹泻持续天数及肠道菌群菌属相对丰度及α多样性指数。结果 两组患儿出生胎龄、出生体重、入组日龄、性别占比、分娩方式、喂养方式、父母乳糖不耐受占比差异均无统计学意义(P> 0.05)。与对照组相比,观察组患儿干预前和干预1 W后每日排便次数差异均无统计学意义(P> 0.05),但观察组患儿较对照组患儿的腹泻天数减少,两组间差异有统计学意义(P=0.004)。与对照组相比,观察组患儿干预1 W后肠道菌群菌属相对丰度和α多样性指数差异均无统计学意义(P> 0.05)。结论 双歧杆菌R0033和R0071联合乳糖酶的干预可能对减少婴儿乳糖不耐受患儿腹泻天数有一定效果,但对肠道菌群组成及多样性无显著影响。Objective To explore the clinical efficacy of bifidobacterium R0033 and R0071 combined with lactase in treating chronic diarrhea due to lactose intolerance among infants,so as to provide new ideas and evidence for clinical treatment.Methods From March 2023 to November 2023,34 infants with lactose intolerance in Chongqing Health Center for Women and Children were recruited as study subjects.They were divided into control and observation group by random number table method,with 17 participants in each group.The control group received lactase treatment only for 1 week,while the observation group received bifidobacterium R0033 and R0071 plus lactase for 1 week.Their general information,the number of bowel movements and the duration of diarrhea pre-and post-intervention of two groups were compared,as well as the relative abundance andα-diversity index of the gut microbiota.Results There were no significant difference in gestational age,birth weight,age at enrollment,gender ratio,mode of delivery,feeding method,and parental lactose intolerance ratio between two groups(P>0.05).Compared with control group,there was no significant difference in terms of the number of daily bowel movements pre-and 1 week post-intervention in the observation group(P>0.05),but the duration of diarrhea in the observation group was significantly shorter than that in the control group(P=0.004).Compared with control group,there was no significant difference in the relative abundance of gut microbiota and theα-diversity index 1 week post-intervention in the observation group(P>0.05).Conclusion The intervention of bifidobacterium R0033 and R0071 combined with lactase may have certain effects on reducing duration of diarrhea among infants with lactose intolerance,but it had no significant effects on the composition and diversity of gut microbiota.

关 键 词：双歧杆菌 乳糖不耐受 婴儿 肠道菌群 

分 类 号：R725.7[医药卫生—儿科]