藏药细果角茴香中16种微量元素含量测定与分析  

Determination and Analysis of 16 Trace Elements in Tibetan Medicine Hypecoum Leptocarpum

作　　者：吴雪花[1] 金秉巾[1] 丁玉 郑心悦 梁永欣 林鹏程 李生有[1] WU Xuehua;JIN Bingjin;DING Yu;ZHENG Xinyue;LIANG Yongxin;LIN Pengcheng;LI Shengyou(Qinghai Provincial People′s Hospital,Xining,Qinghai,China 810007;College of Pharmacy,Qinghai Nationalities University·Qinghai Provincial Key Laboratory for Chemistry of Plant Resources of Qinghai-Tibet Plateau·State Ethnic Affairs Commission Key Laboratory for Protection Development and Utilization of Qinghai-Tibet Plateau Tibetan Medicine Resources,Xining,Qinghai,China 810007)

机构地区：[1]青海省人民医院,青海西宁810007 [2]青海民族大学药学院·青海省青藏高原植物化学重点实验室·国家民委青藏高原藏药资源保护与开发利用重点实验室,青海西宁810007

出　　处：《中国药业》2024年第24期78-83,共6页China Pharmaceuticals

基　　金：青海省科技计划项目[2022-ZJ-Y14];青海省“昆仑英才·高端创新创业人才”专项[青卫健办〔2021〕104号];青海民族大学PI制科研大团队项目[2021XJPI02]。

摘　　要：目的为细果角茴香微量元素含量测定及规范种植提供参考。方法采用电感耦合等离子体发射光谱(ICP-OES)法测定青海省不同产地27批样品中铝(Al)、钡(Ba)、钙(Ca)、钾(K)、镁(Mg)、锰(Mn)、钠(Na)、磷(P)、锶(Sr)、钛(Ti)、硼(B)、铁(Fe)、锂(Li)、钒(V)、锌(Zn)含量,等离子体射频发射功率为1.20 kW,等离子气流量为15 L/min,雾化器流量为0.75 L/min,辅助气流量为1.5 L/min,观察10 min,一次读数3 s,稳定15 s,等离子气、雾化气和辅助气均为高纯氩气,检测波长分别为396.2,455.4,317.9,766.5,279.6,257.6,589.6,213.6,407.8,336.1,249.8,238.2,670.8,292.4,213.9 nm。采用原子荧光光谱法测定硒(Se)含量,灯电流为100 mA,屏蔽气流速为1000 mL/min,负高压为340 V,载气流速为500 mL/min,原子化器高度为8 mm,注入体积为2 mL,载气、屏蔽气均为高纯氩气。采用主成分分析(PCA)和判别对应分析(DCA)各微量元素含量测定结果。结果15种元素和Se质量浓度分别在0.02~10μg/mL、0~40μg/L范围内与峰面积线性关系良好(r>0.9996);检测限分别为0.018~3.000 mg/L、0.002 mg/L;精密度、重复性试验结果的RSD均小于3.0%;平均加样回收率为94.01%~102.15%及91.44%,RSD均小于10%(n=6)。各批样品中16种元素的平均含量排序为K>Ca>Mg>P>Na>Fe>Al>Sr>Zn>Mn>Ba>B>Ti>V>Li>Se。PCA结果显示,Al、Fe、Li、Mn是藏药细果角茴香的特征微量元素。DCA结果显示,Fe,Al,Ca,Ti,Mn,V,Ba含量与海拔呈正相关,与经纬度均呈负相关;K,Zn,Sr,Mg,Na、P含量反之;Se,Li,B含量与海拔、经纬度均无相关性。结论建立的方法操作简便,结果稳定、可靠,可用于细果角茴香微量元素含量测定;青海省不同产地细果角茴香中各微量元素的含量差异较大,与产地海拔和经纬度有一定相关性。Objective To provide a reference for the determination of trace element and standardized cultivation of Hypecoum leptocarpum.Methods The inductively coupled plasma-optical emission spectrometry(ICP-OES)method was used to determine the contents of aluminum(Al),barium(Ba),calcium(Ca),potassium(K),magnesium(Mg),manganese(Mn),sodium(Na),phosphorus(P),strontium(Sr),titanium(Ti),boron(B),iron(Fe),lithium(Li),vanadium(V)and zinc(Zn)in 27 batches of samples from different producing areas in Qinghai Province;the plasma radio frequency power was 1.20 kW,the plasma gas flow rate was 15 L/min,the atomizer flow rate was 0.75 L/min,the auxiliary air flow rate was 1.5 L/min,the observation time was 10 min,the single reading time was 3 s,the stabilization time was 15 s,the plasma gas,atomization gas and auxiliary gas were all high-purity argon,the detection wavelengths were 396.2,455.4,317.9,766.5,279.6,257.6,589.6,213.6,407.8,336.1,249.8,238.2,670.8,292.4,213.9 nm respectively.The atomic fluorescence spectrometry was used to determine the content of selenium(Se),the lamp current was 100 mA,the shielding gas flow rate was 1000 mL/min,the negative high voltage was 340 V,the carrier gas flow rate was 500 mL/min,the atomizer height was 8 mm,the injection volume the 2 mL,the carrier gas and shielding gas were both high-purity argon.Principal component analysis(PCA)and discriminant correspondence analysis(DCA)were used to determine the content of various trace elements.Results The linear ranges of 15 elements and Se were 0.02-10μg/mL and 0-40μg/L respectively(r>0.9996).The limits of detection(LOD)were in the range of 0.018 to 3.000 mg/L,0.002 mg/L respectively.The RSDs of precision and repeatability tests were both lower than 3.0%.The average recovery rates of 15 elements and Se were in the range of 94.01%to 102.15%,91.44%respectively,with the RSDs lower than 10%(n=6).The average content of 16 elements in each batch of samples was ranked as K>Ca>Mg>P>Na>Fe>Al>Sr>Zn>Mn>Ba>B>Ti>V>Li>Se.The PCA showed that Al,Fe,Li and Mn were the cha

关 键 词：细果角茴香 微量元素 含量测定 海拔 经度 纬度 青海省 

分 类 号：R917[医药卫生—药物分析学] R927[医药卫生—药学]