正交试验法优选川香解郁饮提取工艺  

Optimize the Extraction Process of ChuanXiang JieYu Drink Based on Orthogonal Test Method

作　　者：郭妹 辜炜君 黄颖华 刘珂君 张贤华 张景双 GUO Mei;GU Wei-jun;HUANG Ying-hua;LIU Ke-jun;ZHANG Xian-hua;ZHANG Jing-shuang(Xiamen Xianyue Hospital,Xianyue Hospital Affiliated with Xiamen Medical College,Fujian Psychiatric Center,Fujian Clinical Research Center for Mental Disorders,Xiamen 361001,China)

机构地区：[1]厦门市仙岳医院,厦门医学院附属仙岳医院,福建省精神医学中心,福建省精神疾病临床医学研究中心,福建厦门361001

出　　处：《海峡药学》2024年第11期28-33,共6页Strait Pharmaceutical Journal

基　　金：2020年福建省卫生健康中青年骨干人才培养项目(2020GGB065)。

摘　　要：目的 确定指标权重,优选川香解郁饮提取工艺,为川香解郁饮进一步开发研究提供参考依据。方法 以洋川芎内酯A、新橙皮苷、白术内酯Ⅲ和出膏率为评价指标进行评分,结合正交试验结果优化川香解郁饮提取工艺参数。结果 洋川芎内酯A、新橙皮苷、白术内酯Ⅲ分别在80~10000 ng·mL^(-1)、80~10000 ng·mL^(-1)、40~5000 ng·mL^(-1)内呈良好的线性关系(R为0.9945~0.9956);综合打分确定的川香解郁饮最佳提取工艺条件为处方药材加6倍量的水,每次1.5 h,煎煮提取3次。结论 综合评分结果合理,符合中医治疗的整体理念。优选得到的工艺稳定、可行,重复性好,可为后续制备工艺标准提供科学依据。OBJECTIVE The extraction process of ChuanXiang JieYu Drink,which could provide a reference for the further development of ChuanXiang JieYu Drink,was optimised by determining the weights of the indicators.METHODS The indicators included ligustrolactone A,neohesperidin,atractylenolide Ⅲ and paste yield was used to score.And the parameters of ChuanXiang JieYu Drink were optimized by combining with the results of orthogonal experiments.RESULTS The results showed good linearity(R=0.9945-0.9956) within 80-10000 ng·mL~(-1),80-10000 ng·mL~(-1) and 40-5000 ng·mL~(-1) for ligustrolactone A,Neohesperidin and atractylenolide Ⅲ,respectively.And the optimal extraction process conditions,which was determined by comprehensive evaluation according to the weighting coefficients,were as follows:add 6 times the amount of water with the prescribed herbs,decoct and extract for 3 times and each for 1.5 h.CONCLUSION The comprehensive score is reasonable,and it conformed to the overall concept of Chinese medicine treatment.The process obtained by the preferred method was stable,feasible and reproducible,and it could provide scientific basis for the standard of preparation process.

关 键 词：川香解郁饮 提取工艺 多重指标 正交试验 液质联用仪 出膏率 

分 类 号：R927[医药卫生—药学]