基于UPLC-MS/MS法比较不同产地蒲公英叶中15种成分含量  

Determination of 15 Chemical Constituents in Dandelion Leaves from Different Areas of Production Based on UPLC-MS/MS

作　　者：雷京奇 张泽昭 任东 王鑫国 牛丽颖 LEI Jingqi;ZHANG Zezhao;REN Dong;WANG Xinguo;NIU Liying(Hebei University of Chinese Medicine,Shijiazhuang Hebei 050091,China;Hebei Traditional Chinese Medicine Formula Granule Engineering&Technology Innovate Center,Shijiazhuang Hebei 050091,China;Quality Evaluation&Standardization Hebei Province Engineering Research Center of Traditional Chinese Medicine,Shijiazhuang Hebei 050091,China)

机构地区：[1]河北中医药大学,河北石家庄050091 [2]河北省中药配方颗粒技术创新中心,河北石家庄050091 [3]中药材品质评价与标准化河北省工程研究中心,河北石家庄050091

出　　处：《中国药物警戒》2024年第11期1256-1263,共8页Chinese Journal of Pharmacovigilance

基　　金：河北省自然科学基金资助项目(H2022423335);国家中医药管理局科技项目(GZY-KJS-2023-030);河北省中医药管理局科研计划项目(2025075)。

摘　　要：目的建立超高效液相色谱串联三重四极杆质谱(UPLC-MS/MS)法测定蒲公英叶中15种成分含量的方法,结合多元统计分析为蒲公英叶药材质量评价提供参考。方法采用Waters Acquity UPLC^(R)HSS T3色谱柱,以乙腈-0.1%甲酸水为流动相,以多重反应监测(MRM)负离子模式检测,结合聚类分析和最小偏二乘判别分析进行评价。结果15种化学成分在各自范围内线性关系良好(r≥0.9977),精密度和准确性良好,相对标准偏差(RSD)均<5%。聚类分析和OPLS-DA分析均可把样品分为4类,可将菊苣酸、单咖啡酰酒石酸、芦丁、秦皮乙素、金丝桃苷、异槲皮苷和木犀草苷作为蒲公英叶药材质量标志物的选择参考。结论建立的方法准确性好,能够为蒲公英叶药材质量控制提供参考。Objective To establish a UPLC-MS/MS method to determine the contents of 15 components in Dandelion leaves in order to provide a reference for quality evaluation.Methods The Waters Acquity UPLC^(R)HSS T3 Column was used while acetonitrile-0.1%formic acid water served as the mobile phase.Negative electrospray ionization was performed in a multiple reaction monitoring(MRM)mode.Cluster analysis and least partial binary analysis were conducted for evaluation.Results The 15 components had a good linear relationship within their respective range(r≥0.9977),with good precision and accuracy(RSD<5%).The samples could be divided into four categories by clustering and OPLS-DA analysis,and chichoric acid,cafftaric acid,rutin,esculetin,hyperoside,isoquercetin and cynaroside were used as reference for the selection of quality markers in Dandelion leaves.Conclusion This method is accurate enough to provide references for quality control of Dandelion leaves.

关 键 词：蒲公英叶 菊苣酸 单咖啡酰酒石酸 芦丁 秦皮乙素 超高效液相色谱串联三重四极杆质谱 含量测定 质量控制 化学成分 

分 类 号：R927.1[医药卫生—药学]