新生儿呼吸窘迫综合征患者的血清IgG N-糖基化特征  

Characteristics of serum IgG N-glycosylation in neonatal patients with respiratory distress syndrome

作　　者：王英杰 闫茹絮 王猛 陈翰祥 李栋 WANG Yingjie;YAN Ruxu;WANG Meng;CHEN Hanxiang;LI Dong(School of Public Health and Health Management,Shandong First Medical University(Shandong Academy of Medical Sciences),Jinan 250117,China;Department of Clinical Laboratory,The First Affiliated Hospital of Shandong First Medical University(Shandong Academy of Medical Sciences),Jinan 250014,China)

机构地区：[1]山东第一医科大学(山东省医学科学院)公共卫生与健康管理学院,山东济南250117 [2]山东第一医科大学(山东省医学科学院)第一附属医院临床检验科,山东济南250014

出　　处：《山东第一医科大学（山东省医学科学院）学报》2024年第11期653-659,共7页Journal of Shandong First Medical University ＆ Shandong Academy of Medical Sciences

基　　金：国家自然科学基金(81973138);国家自然科学基金青年基金(82002755)。

摘　　要：目的通过分析新生儿呼吸窘迫综合征(neonatal respiratory distress syndrome,NRDS)血清IgG N-糖基化修饰的变化,为NRDS的诊断和治疗提供新的思路。方法于2021年12月至2022年9月期间从泰安妇幼保健医院招募67例NRDS患者和70例同期对照新生儿作为研究对象。采用超高压液相色谱(ultra performance liquid chromatography,UPLC)荧光检测器对研究对象血清进行定量和定性分析,测定IgG N-糖基化修饰水平。采用独立样本t检验、卡方检验、Wilcoxon秩和检验和二元logistic回归分析研究对象基本特征、临床指标及糖基化修饰的差异。结果在临床生化检测指标中,γ-谷氨酰转肽酶(γ-glutamyl transferase,GGT)升高是NRDS患儿发病的独立危险因素之一(OR=1.014,P=0.032)。UPLC分析发现,在24种初始聚糖中,调整协变量GGT后,NRDS患儿GP1、GP13、GP23、GP24的面积百分比高于对照组,GP14低于对照组,差异均有统计学意义(P均<0.05)。在NRDS患儿的衍生指标中,代表双半乳糖基化和双半乳糖核心岩藻糖基化占总IgG N-糖基化比例的G2和FG2均低于对照组,差异均有统计学意义(P均<0.05)。结论GGT升高是发生NRDS的独立危险因素之一,NRDS患儿双半乳糖基化和双半乳糖核心岩藻糖基化水平降低,可能参与了NRDS的发生发展。Objective:Neonatal respiratory distress syndrome(NRDS)is a significant contributor to neonatal mortality,necessitating a thorough investigation into its pathogenesis and the development of more effective clinical diagnostic and treatment strategies.This research aims to provide a fresh perspective on NRDS diagnosis and treatment by examining variations in serum IgG N-glycosylation.Methods:The study,conducted between December 2021 and September 2022 at Tai’an Maternal and Child Health Hospital,enrolled 67 NRDS patients and 70 control neonates who met the diagnostic criteria and inclusion criteria with informed guardian consent.Serum IgG N-glycosylation levels were quantitatively and qualitatively assessed using Ultra Performance Liquid Chromatography(UPLC)with a fluorescence detector.Statistical analyses,including t-tests,chi-square test,Wilcoxon rank sum tests,and binary logistic regression,were utilized to evaluate differences in basic characteristics,clinical indicators,and glycosylation modifications between the study groups.Results:The findings indicated thatγ-Glutamyl Transferase(GGT)levels were an independent risk factor for NRDS occurrence among clinical biochemical indicators(OR=1.014,P=0.032).UPLC analysis revealed significant differences in the area percentages of specific glycans between NRDS children and the control group,even after adjusting for GGT levels.Conclusion:Notably,the study identified lower levels of dicalactosylation and dicalactosylation of core fucosylation in NRDS children,suggesting their potential involvement in NRDS development.In conclusion,elevated GGT levels are associated with an increased risk of NRDS,while alterations in specific glycan structures may play a role in the pathogenesis of NRDS.

关 键 词：新生儿 呼吸窘迫综合征 IgG N-糖基化 

分 类 号：R722.1[医药卫生—儿科]