杵针对DPN大鼠血清炎症因子及坐骨神经病理形态影响的实验研究  

Experimental study of the effects of pestle on serum inflammatory factors and sciatic neuropathologic morphology in DPN rats

作　　者：王小艳 黄亚玲 王芳 翁夕媚[4] 林洋 王寒 WANG Xiaoyan;HUANG Yaling;WANG Fang(Guangan Hospital of Traditional Chinese Medicine,Guangan Sichuan 638300,China;The First Affiliated Hospital of Shantou University School of Medicine,Shantou Guangdong 515000,China;Hospital of Chengdu University of Tardtional Chinese Medicine,Chengdu Sichuan 610072,China)

机构地区：[1]广安市中医医院,四川广安638099 [2]汕头大学医学院第一附属医院,广东汕头515063 [3]成都中医药大学附属医院,四川成都610072 [4]绵阳市中医医院,四川绵阳621053 [5]广安市人民医院,四川广安638000 [6]达州市中心医院,四川达州635000

出　　处：《四川中医》2024年第12期46-50,共5页Journal of Sichuan of Traditional Chinese Medicine

基　　金：四川省中医药管理局科学技术研究专项课题(编号:2023MS502、2023MS466)。

摘　　要：目的:建立糖尿病周围神经病变(DPN)的大鼠实验动物模型,研究杵针治疗对DPN大鼠血清炎症因子水平和坐骨神经病理形态的影响,从炎症的角度探讨杵针对DPN的神经保护机制。方法:将38只SD雄性大鼠随机分为正常组11只和模型组27只。造模成功后通过计算机随机分组法,将模型组剩余24只分为模型对照组(8只)、甲钴胺组(8只)、杵针组(8只),正常组剩余8只大鼠为正常对照组。正常对照组和模型对照组分别给予正常饲料和高脂高糖饲料喂养,不做任何干预措施;甲钴胺组在模型对照组的基础上,每日以甲钴铵片0.25mg/kg剂量溶于蒸馏水灌胃1次,持续4w;杵针组在模型对照组的基础上,持杵针奎星笔依次点叩至阳八阵、命门八阵,持七曜混元杵以分理手法运行河车路命门至长强段,每日一次,每次共30min,连续干预4w。实验期间观察并记录各组大鼠的一般状况;干预期间每周测量大鼠体重、血糖;实验前后测定大鼠足底热痛觉反应阈值(PWT);实验后取大鼠坐骨神经观察形态结构和取血标本检测各组大鼠血清中TNF-α、IL-1β、MCP-1含量。结果:干预后,杵针组、甲钴胺组与模型对照组比较,大鼠PWT均显著降低(P<0.05)、大鼠坐骨神经损害均明显减轻、大鼠血清TNF-α、IL-1β、MCP-1水平均显著下降(P<0.05)。结论:杵针能明显改善坐骨神经病理形态结构,降低血清炎症因子TNF-α、IL-1β、MCP-1水平,表明杵针治疗DPN的有效性及抗炎效应。Objective:The experimental rats model of diabetic peripheral neuropathy(DPN)was established to study the effects of pestle treatment on serum inflammatory factors and sciatic neuropathologic morphology of DPN rats,and to explore the neuroprotective mechanism of pestle treatment on DPN from the perspective of inflammation.Methods:Thirty-eight SD male rats were randomly divided into the normal group(n=11)and the model group(n=27).After successful modeling,the remaining 24rats in the model group were randomly divided into model control group(n=8),mecobalamin group(n=8)and clubbing group(n=8).The remaining 8rats in the normal group were normal control group.Normal control group and model control group were given normal diet and highfat and high-sugar diet,respectively,without any intervention measures.On the basis of the model control group,the mecobalamin group was gavaged with mecobalamin tablets(0.25mg/kg)dissolved in distilled water daily for 4weeks.In the pestle group,on the basis of the intervention in the model control group,the Kuixing pen of pestle was used to tap Zhiyang eight array and Mingmen eight array in turn,and the Qiyao Huanyuan Chu was used to run the Heche Road of Mingmen to Changqiang section,once a day,for 30min each time.The intervention lasted for 4weeks.During the experiment to observe and record each rats general condition,body weight and blood glucose were measured weekly during the intervention period.PWT was measured before and after the experiment.After the experiment,the morphological structure of sciatic nerve was observed and the serum levels of TNF-α,IL-1β and MCP-1were detected.Results:After intervention,the PWT of the pestle group and the mecobalamin group was significantly lower than that of the model control group(P<0.05),sciatic nerve damage was significantly reduced,and the serum levels of TNF-α,IL-1βand MCP-1were significantly decreased(P<0.05).Conclusion:The pestle therapy can obviously improve the pathological morphology of sciatic nerve,and significantly reduce the leve

关 键 词：杵针 糖尿病周围神经病变 炎症因子 病理形态学 

分 类 号：R245.3[医药卫生—针灸推拿学]