穴位埋线联合药浴治疗类风湿关节炎大鼠炎性疼痛的机制研究  

Study on the mechanism of action of acupoint catgut embedding and herbal bath therapy on inflammatory pain in rats with rheumatoid arthritis

作　　者：赵子莹 王金茹 陈佳丽 王光义 ZHAO Ziying;WANG Jinru;CHEN Jiali(Medical College,Guizhou Medical University,Guizhou Province Guiyang 550004,China;Zhijin County Peoples Hospital,Zhijin Guizhou 551700,China)

机构地区：[1]贵州医科大学临床医学院,贵州贵阳550004 [2]织金县人民医院,贵州织金551700 [3]贵州医科大学附属医院,贵州贵阳550004

出　　处：《四川中医》2024年第12期60-64,共5页Journal of Sichuan of Traditional Chinese Medicine

基　　金：吕明庄全国名老中医药专家传承工作室织金工作站(黔中医药函[2023]4号)。

摘　　要：目的:观察穴位埋线联合“通痹方”药浴治疗,对类风湿性关节炎大鼠关节炎症评分(AI)、痛阈值、滑膜组织中NF-κB信号通路相关蛋白、血清中炎症因子的影响,探讨穴位埋线联合“通痹方”药浴治疗类风湿关节炎的可能机制。方法:SD大鼠随机分为正常组、模型组、埋线组、药浴组、联合组,n=8。胶原诱导法建立类风湿性关节炎大鼠模型。穴位组双侧“阳陵泉”“足三里”“肝俞”“肾俞”埋线治疗,14d/1次,治疗3次,共42d。药浴组予以“通痹方”足趾部局部药浴治疗,每日一次,每次25min,共42d。联合组给予联合治疗,方法、疗程同埋线组、药浴组。关节炎症评分(AI)衡量大鼠关节肿胀情况,热水浴甩尾法测定大鼠痛阈值,ELISA法检测大鼠血清中IL-1β、TNF-α、IL-6、PGE2含量,Western blot法检测大鼠滑膜组织中NF-κB、IκB、COX-2蛋白表达情况。结果:与正常组相比较,模型组痛阈值明显降低,AI明显升高(P<0.05)。与模型组比较,埋线组、药浴组、联合组痛阈值明显增高(P<0.05),AI明显降低(P<0.05)。与正常组比较,模型组血清中IL-1β、TNF-α、IL-6、PGE2浓度均明显升高(P<0.05);与模型组比较,埋线组、药浴组、联合组血清中IL-1β、TNF-α、IL-6、PGE2浓度均明显降低(P<0.05);与埋线组和药浴组比较,联合组血清中IL-1β、TNF-α、IL-6、PGE2浓度降低明显(P<0.05)。与正常组比较,模型组滑膜中NF-κB、COX-2表达明显增高(P<0.05),IκB明显降低;与模型组比较,埋线组、药浴组、联合组滑膜中NF-κB、COX-2表达明显降低(P<0.05),IκB明显增高;且与埋线组、药浴组相比较,联合组滑膜中NF-κB、COX-2表达降低更明显,而IκB表达明显增高(P<0.05)。结论:穴位埋线联合“通痹方”药浴治疗能有效降低关节炎大鼠血清中IL-1β、TNF-α、IL-6、PGE2浓度,下调NF-κB、COX-2的表达及上调IκB表达,抑制NF-κB/IκB信号通路的活化,从而有效缓解类�Objective:To observe the effects of acupoint catgut embedding combined with herbal bath therapy of Tongbi prescription on the joint inflammation score(AI),pain threshold,NF-κB signalling pathway-related protein in synovial tissues,and serum inflammatory factors of rheumatoid arthritis rats,and to explore the possible mechanism of rheumatoid arthritis treatment with acupoint burrowing combined with Tongbi prescription herbal bath.Methods:SD rats were randomly divided into normal,model,acupoint group,herbal bath therapy and cupoint catgut embedding combined with herbal bath therapy(combination)groups,n=8,and the collagen induction method was used to establish rheumatoid arthritis rat model.The acupoint group treated with acupoint embedding at“Yanglingquan(GB34)”,“Zusanli(ST36)”,“Ganshu(BL18)”and“Shenshu(BI23)”points.One treatment in 14days and three treatments for a total of 42days.The herbal bath group was given Tongbi prescription herbal bath therapy for toes and feet,once a day,25minutes each time,for a total of 42days.In the combination group,both treatments was given,with the same method and course of treatment as that of the acupoint group and the herbal bath group.The joint inflammation score(AI)was used to measure the swelling of the joints in rats,the pain threshold of rats was determined by hot water bath tail swinging method,the levels of IL-1β,TNF-α,IL-6and PGE2in serum were measured by ELISA,and the protein expression of NF-κB,IκB and COX-2in synovial membrane tissues of rats was measured by westernbot.Results:Compared with the normal group,the pain threshold was significantly lower and the joint score was significantly higher in the model group(P<0.05).Compared with the model group,the pain threshold value of the acupoint group,herbal bath group and combination group was significantly higher(P<0.05),and the joint score was significantly lower(P<0.05).Compared with the normal group,the serum concentrations of IL-1β,TNF-α,IL-6,and PGE2in the model group were significantly higher(P

关 键 词：类风湿关节炎 痛阈 穴位埋线 药浴 NF-κB/IκB/IL-1β/TNF-α/IL-6/PGE2 

分 类 号：R245.91[医药卫生—针灸推拿学]