多种痕量元素对鳗草毒性的半效应浓度研究:以地上部分伸长长度和最大光量子产量为指标  

Study on toxicity by half-effect concentrations of trace ele-ments in eelgrass:aboveground elongation length and maxi-mum light quantum yield as indexes

作　　者：王新华 张玉 刘明杰 徐少春[1,2,3,4] 岳世栋 张晓梅 周毅[1,2,3,4] WANG Xinhua;ZHANG Yu;LIU Mingjie;XU Shaochun;YUE Shidong;ZHANG Xiaomei;ZHOU Yi(CAS Key Laboratory of Marine Ecology and Environmental Sciences,Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China;Laboratory for Marine Ecology and Environmental Science,Qingdao Marine Science and Technology Center,Qingdao 266237,China;Field Scientific Observation and Research Station of Yellow-Bohai Sea Temperate Seagrass Bed Ecosystems,Ministry of Natural Resources,Qingdao 266033,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院海洋研究所海洋生态与环境科学重点实验室,山东青岛266071 [2]青岛海洋科技中心海洋生态与环境科学功能实验室,山东青岛266237 [3]自然资源部黄渤海温带海草床生态系统野外科学观测研究站,山东青岛266033 [4]中国科学院大学,北京100049

出　　处：《海洋科学》2024年第9期1-12,共12页Marine Sciences

基　　金：国家重点研发计划专项(2022YFD2401300);国家自然科学基金项目(32270405;42206142)。

摘　　要：海草床是重要的近岸海洋生态系统之一。痕量元素污染是造成沿海海草床退化的原因之一。鳗草(Zostera marina L.)是温带海域最广泛分布的海草种。本文通过室内模拟实验,以鳗草地上部分伸长长度和最大光量子产量(Fv/Fm)为评价指标,研究了砷、铬、汞、镍、纳米银、锌6种痕量元素胁迫下对鳗草的毒性效应,并确定6种痕量元素对鳗草的半效应浓度(EC50)。结果表明,6种元素均对鳗草地上部分伸长长度和Fv/Fm产生了抑制作用,NaAsO2、Na2CrO4·4H2O、HgCl2、AgNPs、NiSO4·6H2O、ZnSO4·7H2O对鳗草的半效应浓度分别为2.1 mg/L(As:16.0μmol/L)、89.8 mg/L(Cr:384.6μmol/L)、0.65 mg/L(Hg:2.2μmol/L)、8.1 mg/mL(Ag:75.0μmol/L)、3.0×103 mg/L(Ni:1.1×104μmol/L)、7.5×104 mg/L(Zn:2.6×105μmol/L),不同痕量元素对鳗草的毒性大小为Hg>As>AgNPs>Cr>Ni>Zn。本文研究结果将为受损海草床的生态修复及保护管理提供科学依据。Eelgrass has a complete structure consisting of roots,stems,and leaves and completes its entire life cycle in seawater.In this study,the toxic effects of sodium arsenite(NaAsO2),sodium chromate tetrahydrate(Na2CrO4·4H2O),mercury chloride(HgCl2),nano-silver(AgNPs),nickel sulfate hexahydrate(NiSO4·6H2O),and zinc sulfate heptahy-drate(ZnSO4·7H2O)on eelgrass were studied through laboratory simulation experiments.The different concentrations used for inducing stress were NaAsO2(0,1,2,4,and 8 mg/L),Na2CrO4·4H2O(0,20,40,80,and 120 mg/L),HgCl2(0,0.4,0.5,0.6,and 0.7 mg/L),AgNPs(0,1,4,7,and 10 mg/L),NiSO4·6H2O(0,1×103,3×103,5×103,and 10×103 mg/L),and ZnSO4·7H2O(0,0.01×103,0.05×103,0.1×103,0.5×103 mg/L).The partial elongation length and maximum light quantum yield(Fv/Fm)were inhibited by all six elements.The half-effect concentrations of NaAsO2,Na2CrO4·4H2O,HgCl2,AgNPs,NiSO4·6H2O,and ZnSO4·7H2O were 2.1 mg/L(As:16.0μmol/L)and 89.8 mg/L(Cr:384.6μmol/L),0.65 mg/L(Hg:2.2μmol/L),8.1 mg/mL(Ag:75.0μmol/L),3.0×103 mg/L(Ni:1.1×104μmol/L),7.5×104 mg/L(Zn:2.6×105μmol/L).The toxicity was in the order of Hg>As>AgNPs>Cr>Ni>Zn.

关 键 词：鳗草 痕量元素 生长 叶绿素荧光 EC50 

分 类 号：P735[天文地球—海洋生物学]