护心康对缺氧/复氧诱导的H9C2心肌细胞铁死亡和细胞凋亡的影响  

Effect of Huxinkang on ferroptosis and apoptosis of H9C2 cardiomyocytes induced by hypoxia/reoxygenation

作　　者：何霞 常嘉昱 朱筱婧 张婷 庄红 卿俊[1] 陈志红 蒋岱君 颜旭 HE Xia;CHANG Jiayu;ZHU Xiaojing;ZHANG Ting;ZHUANG Hong;QING Jun;CHEN Zhihong;JIANG Daijun;YAN Xu(Hunan University of Chinese Medicine,Changsha 410208,Hunan,China;Hunan Provincial Hospital of Integrated Traditional Chinese and Western Medicine,Changsha 410006,Hunan,China)

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]湖南省中西医结合医院,湖南长沙410006

出　　处：《湖南中医杂志》2024年第11期159-164,共6页Hunan Journal of Traditional Chinese Medicine

基　　金：国家中医药临床优秀人才培养项目(国家中医药教育函[2022]1号);湖南省中医药科研计划重点项目(202024);湖南省卫生健康委员会科研重点项目(C202319018666);湖南省教育厅科学研究青年项目(21B0378);湖南省中医药研究院科研计划重点项目(202104);湖南省长沙市自然科学基金项目(kq2208146);湖南中医药大学研究生创新课题(2022CX193)。

摘　　要：目的:探究护心康含药血清通过调控核因子-E2-相关因子2-谷胱甘肽过氧化物酶4(Nrf2-GPX4)通路对抑制缺氧/复氧(H/R)大鼠H9C2心肌细胞铁死亡和细胞凋亡的影响。方法:构建H/R诱导的大鼠心肌细胞H9C2损伤模型,分为空白组、模型组、12%护心康含药血清组(12%护心康组)、6%护心康含药血清组(6%护心康组)及铁死亡抑制剂组(Fer-1组)。观察细胞凋亡率,丙二醛(MDA)、亚铁离子(Fe2+)、谷胱甘肽(GSH)、N-末端B型利钠肽原(NT-proBNP)水平,谷胱甘肽过氧化物酶4(GPX4)和核因子-E2-相关因子2(Nrf2)的表达。结果:模型组细胞凋亡率,MDA、Fe2+、GSH、NT-proBNP水平,Nrf2、GPX4蛋白表达水平,与空白组比较,差异均有统计学意义(P<0.05);护心康各含药血清组及Fer-1组细胞凋亡率及GSH、MDA、Nrf2蛋白表达水平,12%护心康组Fe2+、NT-proBNP水平,护心康各含药血清组GPX4蛋白表达水平,与模型组比较,差异均有统计学意义(P<0.05)。结论:护心康含药血清能减轻H/R诱导的H9C2心肌细胞铁死亡和凋亡,其机制可能与抑制Fe2+、MDA表达,上调Nrf2和GPX4蛋白表达有关。Objective:To investigate the effect of serum containing Huxinkang in inhibiting the ferroptosis and apoptosis of H9C2 cardiomyocytes induced by hypoxia/reoxygenation(H/R)by regulating the nuclear factor erythroid 2-related factor 2(Nrf2)-glutathione peroxidase 4(GPX4)pathway.Methods:H9C2 rat cardiomyocytes were used to establish a model of H/R-induced injury and were then divided into blank group,model group,12%serum containing Huxinkang group(12%Huxinkang group),6%serum containing Huxinkang group(6%Huxinkang group),and ferropto-sis inhibitor group(Fer-1 group).Cell apoptosis rate was observed,as well as the levels of malondialdehyde(MDA),fer-rous ion(Fe2+),glutathione(GSH),and N-terminal pro-brain natriuretic peptide(NT-proBNP)and the expression levels of GPX4 and Nrf2.Results:There were significant differences between the model group and the blank group in cell apoptosis rate,the levels of MDA,Fe2+,GSH,and NT-proBNP,and the protein expression levels of Nrf2 and GPX4(P<0.05).There were significant differences between the Huxinkang groups/the Fer-1 group and the model group in cell apoptosis rate,the levels of GSH and MDA and the protein expression level of Nrf2;there were significant differences in the levels of Fe2+and NT-proBNP between the 12%Huxinkang group and the model group;there was a significant difference in the protein expression level of GPX4 between the Huxinkang groups and the model group(P<0.05).Conclusion:Serum containing Huxinkang can alleviate ferroptosis and apoptosis of H9C2 cardiomyocytes induced by H/R,possibly by inhibiting the expression of Fe2+and MDA and upregulating the protein expression of Nrf2 and GPX4.

关 键 词：护心康 含药血清 缺氧/复氧损伤 H9C2心肌细胞 铁死亡 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]