生肌创愈膏通过p38MAPK信号通路促进大鼠创面愈合的机制  

Exploring the Mechanism of Shengji Chuangyu Ointment Promting Wound Healing in Rats Based on p38MAPK Signaling Pathway

作　　者：赖沁 刘新红[2] 叶茂[2] 李铭杰 李小龙 LAI Qin;LIU Xinhong;YE Mao;LI Mingjie;LI Xiaolong(Jiangxi University of Chinese Medicine,Nanchang 330004,China;The Affiliated Hospital of Jiangxi University of Chinese Medicine,Nanchang 330006,China;Kaiping Central Hospital of Guangdong Province,Kaiping 529000,China;Shishou People′s Hospital of Jingzhou City,Hubei Province,Jingzhou 434400,China)

机构地区：[1]江西中医药大学,江西南昌330004 [2]江西中医药大学附属医院,江西南昌330006 [3]广东省开平市中心医院,广东开平529000 [4]湖北省荆州市石首市人民医院,湖北荆州434400

出　　处：《中国皮肤性病学杂志》2024年第12期1318-1326,共9页The Chinese Journal of Dermatovenereology

基　　金：江西省中医药管理局科技计划项目(2022A231);江西省青年科学基金项目(20202BABL216067);江西省自然科学基金项目(20232BAB206154)。

摘　　要：目的探讨生肌创愈膏通过p38丝裂原活化蛋白激酶(p38MAPK)信号通路对大鼠炎症性创面的修复作用及其潜在机制。方法将实验大鼠随机分为4组:空白对照组(Control)、模型组(Model)、湿润烧伤膏组(MEBO)和生肌创愈膏组(SJCYO)。构建炎症性创面模型后,分别采用生理盐水纱布、湿润烧伤膏纱条和生肌创愈膏纱条敷于创面,连续换药治疗7 d。通过HE染色观察创面病理变化,ELISA检测TNF-α和IL-6含量,RT-PCR法测定p38MAPK mRNA和MAPK活化蛋白激酶-2(MK2)mRNA表达水平,Western blot检测p38MAPK和MK2蛋白磷酸化水平。结果与对照组相比,模型组在病理形态学上显示出明显的炎性细胞浸润和出血、水肿,TNF-α和IL-6含量显著升高,p38MAPK mRNA和MK2 mRNA表达量上升,p38MAPK和MK2蛋白磷酸化水平升高。相比模型组,生肌创愈膏组炎性细胞浸润减少,新生表皮生长,创面愈合加快;TNF-α和IL-6含量显著降低,p38MAPK mRNA和MK2 mRNA表达量降低,p38MAPK和MK2蛋白磷酸化水平下降,且较湿润烧伤膏组有更显著的改善。结论生肌创愈膏通过降低炎症因子TNF-α和IL-6水平,抑制p38MAPK和MK2的mRNA及蛋白表达,有效促进创面愈合。Objective To investigate the reparative effect of Shengji Chuangyu Ointment on inflammatory wounds of rats and its underlying mechanism through the p38MAPK signaling pathway.Methods Rats were randomly divided into four groups:Control,Model,MEBO,and Shengji Chuangyu Ointment(SJCYO).After constructing inflammatory wound models,we applied physiological saline gauze,MEBO gauze,and SJCYO gauze on the wounds respectively for 7 days.Pathological changes were observed by HE staining,TNF-αand IL-6 levels were measured by ELISA,p38MAPK mRNA and MK2 mRNA expression levels were determined by RT-PCR,and the phosphorylation levels of p38MAPK and MK2 proteins were detected by Western blot.Results Compared with the control group,the model group showed significant inflammatory cell infiltration,bleeding,and edema,with markedly increased levels of TNF-αand IL-6,upregulated expression of p38MAPK mRNA and MK2 mRNA,and elevated phosphorylation levels of p38MAPK and MK2 proteins.Compared with the model group,the SJCYO group had reduced inflammatory cell infiltration,increased new epidermal growth,and accelerated wound healing;TNF-αand IL-6 levels were significantly decreased,p38MAPK mRNA and MK2 mRNA expression levels were reduced,and the phosphorylation levels of p38MAPK and MK2 proteins were decreased,showing more significant improvement compared to the MEBO group.Conclusion Shengji Chuangyu Ointment effectively promotes wound healing by reducing the levels of inflammatory factors TNF-αand IL-6 and inhibiting the expression of p38MAPK and MK2 mRNA and proteins.

关 键 词：生肌创愈膏 P38MAPK信号通路 炎症 创面愈合 磷酸化 

分 类 号：R751[医药卫生—皮肤病学与性病学]