复方枸杞熏蒸制剂超声雾化治疗大鼠蒸发过强型干眼模型的实验观察  

Experimental Observation of Ultrasonic Nebulization with Compound Lycii Fructus Fumigation Preparation in the Treatment of Hyperevaporative Dry Eye Model in Rats

作　　者：方娜 龚佳怡[1] FANG Na;GONG Jiayi(Suzhou Hospital of Traditional Chinese Medicine Affiliated to Nanjing University of Chinese Medicine,Suzhou 215009,China)

机构地区：[1]南京中医药大学附属苏州市中医医院,江苏苏州215009

出　　处：《中医药信息》2024年第12期17-22,共6页Information on Traditional Chinese Medicine

基　　金：江苏省苏州市科技局指导性项目(SKJYD2021127)。

摘　　要：目的:探讨复方枸杞熏蒸制剂超声雾化治疗对大鼠蒸发过强型干眼模型角膜状态及泪膜质量的影响。方法:40只清洁级SD雄性大鼠80只眼随机分为4组,即对照组、模型组、玻璃酸钠滴眼组和中药雾化组。除对照组外,其余3组通过机械性阻止瞬目法诱导蒸发过强型干眼模型。造模成功后,模型组不做处理;玻璃酸钠滴眼组给予玻璃酸钠滴眼液每日3次,每次1滴;中药雾化组给予复方枸杞熏蒸制剂雾化,每日2次,每次20 min。分别于干预前、干预2 d、干预1周和干预2周时观察各组大鼠泪膜破裂时间、角膜损伤评分、泪液pH值等指标。结果:中药雾化2 d后,泪膜破裂时间在中药雾化组和玻璃酸钠滴眼组中明显延长,与模型组比较差异显著(P<0.001);玻璃酸钠滴眼组和中药雾化组分别于1周和2周后无明显差异;中药雾化组治疗的效果在2 d后最为显著,泪膜破裂时间基本接近对照组水平。中药雾化2 d后,与模型组比较,中药雾化组角膜损伤评分显著下降,而玻璃酸钠滴眼组则无显著改变;1周和2周后,中药雾化组和玻璃酸钠滴眼组的角膜损伤评分均显著降低(P<0.001),中药雾化组改善更为显著。中药雾化2 d后,与模型组比较,中药雾化组泪液pH值显著降低,而玻璃酸钠滴眼组则无明显变化;1周和2周后,中药雾化组泪液pH值继续降低,而玻璃酸钠滴眼组无明显变化;中药雾化组治疗的效果在2周后最为显著,其泪液pH值降低更为显著。结论:复方枸杞熏蒸制剂雾化治疗在延长干眼症患者泪膜破裂时间、改善角膜损伤评分和降低泪液pH值方面效果明显,比玻璃酸钠滴眼治疗疗效更好。Objective:To explore the effects of ultrasonic nebulization with Compound Lycii Fructus Fumigation Preparation on corneal condition and tear film quality in a rat model of hyperevaporative dry eye.Methods:Forty clean-grade SD male rats(80 eyes)were randomly divided into four groups:control group,model group,sodium hyaluronate eye drop group,and herbal nebulization group.All groups except the control group were induced into an hyperevaporative dry eye model by mechanical prevention of blinking.After successful modeling,the model group received no treatment;the sodium hyaluronate eye drop group received sodium hyaluronate eye drops,three times daily,one drop per time;the herbal nebulization group received Compound Lycii Fructus Fumigation Preparation nebulization twice daily,20 minutes each time.Observations of tear film break-up time,corneal injury scores,and tear pH were conducted before intervention,after 2 days of intervention,after 1 week,and after 2 weeks.Results:Tear Film Break-up Time:After 2 days of herbal nebulization,tear film break-up time significantly increased in both herbal nebulization and sodium hyaluronate eye drop groups compared with the model group(P<0.001).No significant differences were observed between the sodium hyaluronate eye drop group and herbal nebulization group at 1 and 2 weeks.The most significant effect of the herbal nebulization treatment was observed after 2 days,with tear film break-up time close to that of the control group.Corneal Injury Score:After 2 days of herbal nebulization,the corneal injury score significantly decreased in the herbal nebulization group compared to the model group,with no significant change in the sodium hyaluronate eye drop group.Both herbal nebulization and sodium hyaluronate groups showed significantly reduced corneal injury scores at 1 and 2 weeks(P<0.001),with herbal nebulization group showing greater improvement.Tear pH:After 2 days of herbal nebulization,tear pH significantly decreased in the herbal nebulization group compared to the model group,

关 键 词：复方枸杞熏蒸制剂 蒸发过强型干眼 泪液pH 角膜上皮损伤 

分 类 号：R285.5[医药卫生—中药学]