健胃止泻合剂对脾虚型功能性腹泻大鼠脑肠肽VIP、CCK表达的影响  

Effect of Jianwei Zhixie Mixture on the Expression of Brain Gut Peptides VIP and CCK in Rats with Functional Diarrhea of Spleen Deficiency Type

作　　者：武正权[1] 杨红娟 姚柳伊[1] 郭军[1] WU Zhengquan;YANG Hongjuan;YAO Liuyi;GUO Jun(Gansu Provincial Hospital of Traditional Chinese Medicine,Lanzhou 730050,China;Gansu University of Traditional Chinese Medicine,Lanzhou 730000,China)

机构地区：[1]甘肃省中医院,甘肃兰州730050 [2]甘肃中医药大学,甘肃兰州730000

出　　处：《中医药信息》2024年第12期23-26,31,共5页Information on Traditional Chinese Medicine

基　　金：甘肃省自然科学基金项目(21JR11RA206)。

摘　　要：目的:观察不同剂量健胃止泻合剂对脾虚型功能性腹泻大鼠脑肠肽VIP、CCK表达的影响。方法:50只SPF级Wistar大鼠随机分为空白对照组、模型组、妈咪爱组、健胃止泻合剂低剂量组、健胃止泻合剂高剂量组各10只。除空白对照组外,其余各组大鼠采用灌胃大黄水煎液7.5 g/(kg·d),并每日负重游泳,以力竭为度的方法建立脾虚型功能性腹泻模型。在造模的同时,模型组和空白对照组每天给予大鼠自由饮用0.9%NaCl溶液,健胃止泻合剂高、低剂量组分别灌胃健胃止泻合剂18、4.5 g/(kg·d),妈咪爱组灌胃妈咪爱0.2 g/(kg·d),连续14 d。给药结束后,脱颈处死大鼠,免疫组化法测定大鼠血清中VIP、CCK的表达。结果:与空白对照组比较,模型组脑肠肽VIP、CCK表达显著增加(P<0.05)。与模型组比较,妈咪爱组脑肠肽VIP、CCK表达降低,健胃止泻合剂低、高剂量组脑肠肽VIP表达显著降低(P<0.05),健胃止泻合剂低、高剂量组脑肠肽CCK表达显著降低(P<0.05)。结论:健胃止泻合剂可通过降低脾虚型功能性腹泻大鼠脑肠肽VIP、CCK的表达,调节肠道运动和水液代谢,从而改善功能性腹泻。Objective:To observe the effects of different doses of Jianwei Zhixie Mixture on the expression of cerebral intestinal peptide VIP and CCK in rats with functional diarrhea with spleen deficiency.Methods:50 SPF rats were randomly divided into blank control group,model group,Mommy love group,Jianwei Zhixie Mixture low-dose group and Jianwei Zhixie Mixture high-dose group with 10 rats each.The Mamai group was given 0.2g/(kg·d)intragastric administration,and the low and high dose Jianwei Zhixie Mixture groups were given 4.5 and 18g/(kg·d)Jianwei Zhixie Mixture intragastric administration,respectively,for a total of 14 days.The rats in the model group and the blank control group were given 0.9%NaCl solution freely every day for 14 days.After administration,the rats were killed by neck removal,and the expressions of VIP and CCK in serum were determined by immunohistochemistry.Results:Compared with blank control group,the expressions of VIP and CCK were significantly increased in model group(P<0.05).Compared with the model group,the expression of cerebral intestinal peptide VIP and CCK decreased in the mommy love group.Compared with model group,VIP expression of brain-gut peptide was significantly decreased in low-dose and high-dose Jianwei Zhixie Mixture groups(P<0.05),CCK expression of brain-gut peptide was significantly decreased in high-dose Jianwei Zhixie Mixture group(P<0.05).Conclusion:Jianwei Zhixie Mixture can improve functional diarrhea by reducing the expression of VIP and CCK,regulating intestinal movement and water metabolism in rats with functional diarrhea of spleen deficiency.

关 键 词：健胃止泻合剂 脾虚型功能性腹泻 VIP CCK 

分 类 号：R285.5[医药卫生—中药学]