26%环磺酮·烟嘧磺隆·特丁津OD HPLC分析方法研究  

Research on the HPLC analytical method for tembotrione·nicosulfuron·terbuthylazine 26%OD

作　　者：杨柳 郭亚虎 王海朋 姜万年 耿彪 王向前 徐梁 YANG Liu;GUO Yahu;WANG Haipeng;JIANG Wannian;GENG Biao;WANG Xiangqian;XU Liang(Shandong Vicome Greenland Chemical Co.,Ltd.,Jinan 250204,China;Beijing DaBeiNong Biotechnology Co.,Ltd.,Beijing 100194,China)

机构地区：[1]山东胜邦绿野化学有限公司,济南250204 [2]北京大北农生物技术有限公司,北京100194

出　　处：《世界农药》2024年第12期50-54,共5页World Pesticide

摘　　要：采用高效液相色谱分析方法(HPLC),以乙腈+磷酸水溶液(pH 3.0)为流动相,使用C_(18)不锈钢柱和二极管阵列检测器,在235 nm波长,对26%环磺酮·烟嘧磺隆·特丁津可分散油悬浮剂(OD)产品中有效成分环磺酮、烟嘧磺隆和特丁津的含量进行了分离和定量分析。结果表明,在该色谱条件下烟嘧磺隆、环磺酮和特丁津的质量浓度与其相应峰面积响应值之间呈现出良好的线性相关,其回归方程分别为y=96525.755x+5.2106、y=38721.7727x+3.5122和y=97558.0497x+6.9862,相关系数均为0.9999;5批次样品中烟嘧磺隆、环磺酮和特丁津含量测定结果的标准偏差分别为0.01、0.03、0.08;变异系数分别为0.54%、0.75%、0.39%;空白样品添加烟嘧磺隆、环磺酮和特丁津标样,其平均回收率分别为99.80%、99.73%、99.94%。说明该方法可适用于26%环磺酮·烟嘧磺隆·特丁津OD产品生产过程的质量控制要求。The contents of tembotrione,nicosulfuron and terbuthylazine in tembotrione·nicosulfuron·terbuthylazine 26%OD were separated and quantitatively analyzed by HPLC with acetonitrile and phosphoric acid aqueous solution(pH 3.0)as the mobile phase,a C_(18)stainless steel column and a diode array detector at a wavelength of 235 nm.The results showed that under the chromatographic conditions,there was a good linear correlation between the mass concentrations of nicosulfuron,tembotrione and terbuthylazine and their corresponding peak area response values.The regression equations were y=96525.755x+5.2106,y=38721.7727x+3.5122 and y=97558.0497x+6.9862,respectively,and the correlation coefficients were all 0.9999.The standard deviations of the determination results of nicosulfuron,tembotrione and terbuthylazine in 5 batches of samples were 0.01,0.03 and 0.08,respectively;the coefficients of variation were 0.54%,0.75%and 0.39%,respectively;the average recoveries of the blank samples added with nicosulfuron,tembotrione and terbuthylazine standards were 99.80%,99.73%and 99.94%,respectively.This indicates that this method is applicable to the quality control requirements of the production process of tembotrione·nicosulfuron·tembotrione 26%OD products.

关 键 词：26%环磺酮·烟嘧磺隆·特丁津OD 高效液相色谱 定量分析 

分 类 号：TQ457[化学工程—农药化工]