人工诱导细鳞鱼三倍体的研究  

Artificial induction of triploidy in Brachymystax lenok

作　　者：孙丽莹 马瑞祥 王茂林[1,2] 文逊智 SUN Liying;MA Ruixiang;WANG Maolin;WEN Xunzhi(Key Laboratory of Mariculture,Ministry of Agriculture and Rural Affairs,Dalian Ocean University,Dalian 116023,China;Northern Key Laboratory of Applied Biology and Enhancement of Fish Culture,Liaoning Province,Dalian 116023,China)

机构地区：[1]大连海洋大学农业农村部海水增养殖学重点实验室,辽宁大连116023 [2]辽宁省北方鱼类应用生物学与增养殖重点实验室,辽宁大连116023

出　　处：《黑龙江动物繁殖》2024年第6期42-45,共4页Heilongjiang journal of animal reproduction

基　　金：2021年辽宁省海洋经济发展项目;辽宁省教育厅服务地方项目(DL201703)。

摘　　要：为探索细鳞鱼(Brachymystax lenok)三倍体育种技术,试验以健康性成熟的细鳞鱼亲鱼为材料,采用热休克法培育细鳞鱼三倍体个体,即采集其鱼卵分为试验组和对照组,分别进行人工授精,试验组按照处理温度26℃、28℃、30℃,受精后起始处理时间10,15,20 min,处理时长10,15,20 min的条件进行热休克处理,对照组不进行热休克处理,测定各组受精率、孵化率和三倍体诱导率。结果表明:试验组的受精率都在93.00%以上,平均孵化率在34.5%左右;在热休克处理温度28℃、受精后起始处理时间10 min、处理时长15 min的条件下三倍体诱导率最高,为100%。说明适宜的热休克处理温度、起始时间及处理时长对细鳞鱼三倍体的形成十分重要。In order to explore the triploid breeding technology of Brachymystax lenok,the collected eggs from healthy mature parent fish of Brachymystax lenok were divided into an experimental group and a control group.After artificial insemination,the experimental group was treated with heat shock for a duration of 10,15,and 20 min at the temperatures of 26℃,28℃,and 30℃,at 10,15,and 20 min after fertilization,and no heat shock for the control group,from which,the fertilization rate,hatching rate,and triploid rate of each group were evaluated.The results showed that the fertilization rate of the experimental group was over 93.00%,and the average hatching rate was 37.5%;The triploid yield was the highest(100%),when a 28℃heat shock was applied for 15 min,10 min after fertilization,suggesting the significance of the appropriate temperature,suitable treatment timing after fertilization and duration of heat shock for the induction of triploidy in Brachymystax lenok.

关 键 词：细鳞鱼(Brachymystax lenok) 热休克蛋白(HSP) 三倍体诱导率 受精率 处理温度 

分 类 号：S961.6[农业科学—水产养殖]