自拟乳康方剂指纹图谱建立及刺桐碱含量测定  

Establishment of Fingerprint Chromatography and Determination of L-Hypaphorine Content in Self-Developed Rukang Formula

作　　者：刘雪松 张艳 薛沾枚 钟鹏 邱景会 孟维珊 李伟 汤继龙 杨庆稳[3] 谭素雯[3] 史同瑞 LIU Xuesong;ZHANG Yan;XUE Zhanmei;ZHONG Peng;QIU Jinghui;MENG Weishan;LI Wei;TANG Jilong;YANG Qingwen;TAN Suwen;SHI Tongrui(Heilongjiang Key Laboratory of Veterinary Drugs,Animal Husbandry and Veterinary Institute,Heilongjiang Academy of Agricultural Science,Qiqihar 161005,China;Heilongjiang Lingkang Biotechnology Co.,Ltd.,Qiqihar 161200,China;Department of Animal Science and Technology,Chongqing Three Gorges Vocational College,Wanzhou 404155,China)

机构地区：[1]黑龙江省农业科学院畜牧兽医分院黑龙江省兽用药物重点实验室,黑龙江齐齐哈尔161005 [2]黑龙江领康生物科技有限公司,黑龙江齐齐哈尔161200 [3]重庆三峡职业学院动物科技学院,重庆万州404155

出　　处：《中国兽医杂志》2024年第12期123-132,共10页Chinese Journal of Veterinary Medicine

基　　金：黑龙江省重点研发计划项目(GA21B006);黑龙江省应用技术研究与开发计划项目(GA20B201);黑龙江省省属科研院所科研业务费项目(CZKYF2022-1-C010);黑龙江省农业科学院畜牧兽医分院2022年自拟课题(ZNKT-202207);重庆市教委科学技术研究项目(KJQN202203511)。

摘　　要：为建立乳康方剂中药提取液(王不留行、甘草、蒲公英、黄芪、连翘和当归)高效液相色谱(HPLC)指纹图谱并测定提取液中刺桐碱含量,结合化学模式识别法评价乳康方剂的质量。本试验应用《中药色谱指纹图谱相似度评价系统》(2012年版)绘制10批次乳康方剂提取液的HPLC指纹图谱进行相似度评价,并确认共有峰;应用SPSS 26.0软件的聚类分析以及SIMCA 14.1软件的主成分分析和正交偏最小二乘法判别分析对10批次乳康方剂提取液指纹图谱进行质量评价,筛选引起组间差异的标志物;使用本试验所建立刺桐碱HPLC检测方法检测不同批次乳康方剂提取液中刺桐碱含量。结果显示,10批次乳康方剂提取液中共有28个共有峰,相似度介于0.903~0.938;聚类分析、主成分分析和正交偏最小二乘法判别分析均可将10批乳康方剂提取液分为3类,且分类结果一致;通过正交偏最小二乘法判别分析筛选出可导致组间差异的9个共有峰;使用所建立的刺桐碱HPLC检测方法测得10批次乳康方剂提取液中刺桐碱平均含量为0.0189 mg/mL。结果表明,本试验建立的HPLC指纹图谱分析方法操作简便、检测结果准确,结合多元统计分析可为自拟乳康方剂的质量控制提供科学依据。To establish a high-performance liquid chromatography(HPLC)fingerprint for the herbal extract of Rukang formula(consisting of Vaccaria Semen,Radix Glycyrrhizae,Taraxacum mongolicum Hand.-Mazz,Astragali Radix,Forsythia fructu,Angelica Sinensis Radix)and to determine the L-hypaphorine content within the extract,this study combines chemical pattern recognition methods to evaluate the quality of the Rukang formula.The HPLC fingerprint of 10 batches of Rukang formula extract was generated and similarity evaluation was conducted using the"Traditional Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System"(2012 version),identifying common peaks.The fingerprint of 10 batches of Rukang formula extract was further assessed for quality through cluster analysis using SPSS 26.0 and principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA)with SIMCA 14.1 software to identify markers responsible for differences among groups.The HPLC method established in this study was used to determine the L-hypaphorine content in different batches of Rukang formula extract.The results showed that there were 28 common peaks in the HPLC fingerprints of the 10 batches of extracts,with similarity values ranging from 0.903 to 0.938.Cluster analysis,PCA,and OPLS-DA all classified the 10 batches into 3 categories with consistent results;OPLS-DA identified 9 common peaks contributing to intergroup differences.The established HPLC method detected an average L-hypaphorine content of 0.0189 mg/mL in the 10 batches.The findings indicate that the HPLC fingerprint analysis method established in this study is simple to perform and provides accurate results,and that multivariate statistical analysis offers a scientific basis for quality control of the self-developed Rukang formula.

关 键 词：催乳 中药 指纹图谱 刺桐碱 含量测定 

分 类 号：S853.7[农业科学—临床兽医学]