基于网络药理学探究积雪草酸联合木犀草素抗宫颈癌的作用机制  

Exploring the Mechanism of Asiatic Acid Combined with Luteolin in Anti-Cervical Cancer Based on Network Pharmacology

作　　者：张军丹 秦海霞[1] 李华伟 张运峰[3] ZHANG Jun-dan;QIN Hai-xia;LI Hua-wei;ZHANG Yun-feng(Department of Obstetrics and Gynecology,The First Affilated Hospital of Xinxiang Medical University,Xinxiang 453100,China;Department of Scientific Research,Tangshan Normal University,Tangshan 063000,China;Department of Life Sciences,Tangshan Normal University,Tangshan 063000,China)

机构地区：[1]新乡医学院第一附属医院妇产科,河南新乡453100 [2]唐山师范学院科研处,河北唐山063000 [3]唐山师范学院生命科学系,河北唐山063000

出　　处：《唐山师范学院学报》2024年第6期51-55,共5页Journal of Tangshan Normal University

基　　金：河南省高等学校重点科研项目计划(19A320030);河北省自然科学基金项目(B2022105015);校企合作项目(2023HX05)。

摘　　要：通过Pubchem、ETCM、SuperPred、SwissTargetPrediction和GEO数据库获取积雪草酸、木犀草素、宫颈癌的交集靶点,构建蛋白互作网络图,并进行GO功能和KEGG通路富集分析。采用Cytoscape软件构建“药物-靶点-通路”网络机制图并进行分子对接。结果发现积雪草酸、木犀草素、宫颈癌共133个交集靶点、299项GO功能和60条KEGG信号通路,6个核心靶点(IL1B、CXCL8、CASP8、CCL2、MMP9、PRKCB),分子对接的结合能均<-5.0kcal·mol^(-1),说明积雪草酸联合木犀草素能够通过多靶点、多通路发挥抗宫颈癌的作用。Intersection targets for asiatic acid,luteolin,and cervical cancer were obtained from Pub-Chem,ETCM,SuperPred,SwissTargetPrediction,and GEO databases,protein-protein interaction network was constructed,and GO functional and KEGG pathway enrichment analyses were performed.The"Drug-Target-Pathway"network mechanism diagram was built using Cytoscape software,and conducted molecular docking.Results revealed 133 intersecting targets for asiatic acid,luteolin,and cervical cancer,involving 299 GO functions and 60 KEGG signaling pathways,with six core targets identified(IL1B,CX-CL8,CASP8,CCL2,MMP9,and PRKCB).Molecular docking binding energies were less than-5.0 kcal·mol^(-1).The results indicate that asiatic acid combined with luteolin can exert anti-cervical cancer effects through multiple targets and pathways.

关 键 词：积雪草酸 木犀草素 宫颈癌 网络药理学 作用机制 

分 类 号：R711[医药卫生—妇产科学]