基于形态特征、COI基因序列和杂交试验的云南茶园小绿叶蝉种类鉴定  

Species identification of tea green leafhoppers in tea gardens of Yunnan,based on morphological characteristics,COI gene sequence and hybridization experiment

作　　者：孟召娜 楚博 罗宗秀[1] 李兆群[1] 边磊[1] 修春丽 付楠霞 赵远艳 胡艳萍 陈宗懋[1] 蔡晓明[1] MENG Zhao-Na;CHU Bo;LUO Zong-Xiu;LI Zhao-Qun;BIAN Lei;XIU Chun-Li;FU Nan-Xia;ZHAO Yuan-Yan;HU Yan-Ping;CHEN Zong-Mao;CAI Xiao-Ming(Tea Research Institute,Chinese Academy of Agricultural Sciences,Hangzhou 310008,China;College of Plant Protection,Henan Agricultural University,Zhengzhou 450046,China;Pu′er Tea Research Institute,Pu′er 665000,China)

机构地区：[1]中国农业科学院茶叶研究所,杭州310008 [2]河南农业大学植物保护学院,郑州450046 [3]普洱市茶叶科学研究所,普洱665000

出　　处：《昆虫学报》2024年第11期1525-1534,共10页Acta Entomologica Sinica

基　　金：国家茶叶产业技术体系(CARS-19);浙江省自然科学基金项目(LY24C140002);云南省重大科技专项计划(202402AE090015)。

摘　　要：【目的】前期研究发现,云南普洱茶园的小绿叶蝉与我国其他产茶省茶园的小贯小绿叶蝉Empoasca(Matsumurasca)onukii形态相似,但阳茎形态略有差别。本研究旨在明确云南茶园发生的小绿叶蝉是否为小贯小绿叶蝉,以期为云南茶园小绿叶蝉防治提供科学依据。【方法】采集了云南4市11区/县13个茶园的小绿叶蝉样品和贵州省1个茶园、浙江省2个茶园的小贯小绿叶蝉样品。通过形态学观察、COI基因序列比对和杂交试验,以明确云南省茶园发生的小绿叶蝉种类。【结果】云南省13个茶园采集的小绿叶蝉样品雄虫阳茎干基腹面均具一刺突,而贵州和浙江省小贯小绿叶蝉样品无此特征。云南13个茶园的小绿叶蝉COI基因序列NCBI上的搜索结果与E.(M.)onukii的COI序列的一致性在99.35%~99.74%;16个采样地的小绿叶蝉遗传距离两两比对在0.000~0.011;单倍型丰度聚类分析显示,云南的样品单独成支,贵州和浙江的样品聚集为一支。云南普洱茶园小绿叶蝉的雌雄成虫与浙江杭州小贯小绿叶蝉可自然发生交配,并产生可育的F_(1)代。两种群间无生殖隔离。正反杂交F_(1)代的孵化率、羽化率、若虫历期无显著差异。F_(1)自交的F 2代其单雌产卵量、羽化率、雌雄比和若虫历期无显著差异。【结论】云南茶园发生的小绿叶蝉为小贯小绿叶蝉E.(M.)onukii,我国茶园中的小贯小绿叶蝉已经分化出不同形态特征的地理种群。【Aim】The previous studies demonstrated that there was a slight difference in aedeagus morphology between the tea green leafhopper from Pu′er tea gardens in Yunnan Province,Southwest China and Empoasca(Matsumurasca)onukii from tea gardens in other provinces although the highly similar morphological characteristics.In order to provide a scientific basis for the control of the tea green leafhoppers in Yunnan,this study was carried out to determine whether the species of the tea green leafhoppers occurring in Yunnan tea gardens is E.(M.)onukii.【Methods】Tea green leafhopper samples were collected from 13 tea gardens in 11 districts/counties of four cities in Yunnan Province,and E.(M.)onukii samples were collected from one tea garden in Guizhou Province and two tea gardens in Zhejiang Province.The morphological observations,comparisons of the COI gene sequences,and hybridization experiments were used to identify the species of the tea green leafhoppers in Yunnan tea gardens.【Results】The ventro-basal side of the male aedeagal shaft of the tea green leafhoppers from 13 tea gardens in Yunnan had a spiny protuberance,but there was no such characteristic in E.(M.)onukii samples from Guizhou and Zhejiang.The COI gene sequences of tea green leafhoppers from 13 tea gardens in Yunnan showed 99.35%-99.74%identity with those of E.(M.)onukii on NCBI.The pairwise genetic distance of tea green leafhoppers from 16 collecting localities ranged from 0.000 to 0.011.The cluster analysis of haplotype abundance showed that the samples from Yunnan formed a separate branch while those from Guizhou and Zhejiang formed a branch.In the hybridization experiments,both female and male adults of Pu′er population in Yunnan could naturally mate with E.(M.)onukii from Hangzhou,Zhejiang,and produced the fertile F_(1)generation.There was no reproductive isolation between the two populations.The hatching rates,emergence rates,and nymphal duration among the F_(1)generation from hybridization experiments showed no significant difference.Amo

关 键 词：茶小绿叶蝉 云南 雄性生殖器 COI 杂交 

分 类 号：Q969[生物学—昆虫学]