饲粮添加高剂量铁对22-42日龄黄羽肉鸡生长性能和空肠黏膜氧化应激的影响  

Effects of High Dosage Dietary Iron on Growth Performance and Oxidative Stress of Jejunal Mucosal in Yellowfeathered Chickens Aged 22 to 42 Days

作　　者：叶金玲 范秋丽 林厦菁 罗琦丽 陈利民 苟钟勇 蒋守群 YE Jinling;FAN Qiuli;LIN Xiajing;LUO Qili;CHEN Limin;GOU Zhongyong;JIANG Shouqun(Institute of Animal Science,Guangdong Academy of Agricultural Sciences,State Key Laboratory of Swine and Poultry Breeding Industry,Key Laboratory of Animal Nutrition and Feed Science in South China,Ministry of Agriculture and Rural Affairs,Guangdong Public Laboratory of Animal Breeding and Nutrition,Guangdong Key Laboratory of Animal Breeding and Nutrition,Guangzhou Guangdong 510640)

机构地区：[1]广东省农业科学院动物科学研究所/猪禽种业全国重点实验室/农业部农村部华南动物营养与饲料重点实验室/广东省畜禽育种与营养研究重点实验室,广东广州510640

出　　处：《广东畜牧兽医科技》2024年第6期1-7,共7页Guangdong Journal of Animal and Veterinary Science

基　　金：国家重点研发计划项目(2021YFD1300404、2022YFD1300502);广东省自然科学基金项目(2022A1515012069);现代农业产业技术体系国家肉鸡产业技术体系(CARS-41);广州市科技计划项目(202206010168、2024E04J0562);广东省基础与应用基础研究基金项目(2024A1515012121);广东省农业科学院科技计划项目(202106TD);广东省农业科学院科技人才引进专项资金项目(R2021YJ-YB3012);广东省农业科学院青年科技人员指导协议(R2021QD-024);广东省畜禽育种与营养研究重点实验室开放课题(2021SZ03)。

摘　　要：该试验旨在研究饲粮添加高剂量铁对22-42日龄黄羽肉鸡的生长性能和空肠黏膜氧化应激的影响。选用健康、发育良好的22日龄快大型岭南黄羽肉公鸡450只,按体重一致原则随机分成3个处理组,每处理6重复,每重复25只鸡。基础饲粮中分别添加0(对照组)、1200 mg/kg和2400 mg/kg葡萄糖酸亚铁(以Fe计)。试验期21 d,试验期间试鸡自由采食与饮水。结果显示,三个处理组试鸡的平均日采食量、平均日增重和料重比均没有显著差异(P>0.05),1200 mg/kg和2400 mg/kg葡萄糖酸亚铁组的腹泻率显著高于对照组(P<0.05)。2400 mg/kg葡萄糖酸亚铁组的血浆诱导型一氧化氮合酶(iNOS)和肌酸激酶(CK)的活性以及空肠黏膜中的丙二醛(MDA)含量显著高于对照组(P<0.05)。1200 mg/kg和2400 mg/kg葡萄糖酸亚铁组空肠组织中的核因子E2相关因子2(Nrf2)、尾侧型同源转录因子2(CDX2)和闭合小环蛋白-1(ZO-1)基因表达量显著低于对照组(P<0.05)。综合以上所有试验结果表明,饲粮中添加高剂量(1200-2400 mg/kg)的葡萄糖酸亚铁对22-42日龄黄羽肉鸡生长性能虽无负面影响,但却诱导了肉鸡空肠黏膜氧化损伤,且损伤程度与葡萄糖酸亚铁添加剂量有关,进而导致腹泻率显著提高。This experiment was conducted to study the effects high dosage dietary iron ingested on growth performance and jejunal mucosal oxidative stress of Yellow-feathered chickens aged 22 to 42 days.A total of 45022-day-old male rapidly-growing Yellow-feathered chickens were randomly divided into 3 groups with 6 replicates per group and 25 birds per replicate.The basal diets were supplemented with 0(control group),1200 mg/kg and 2400 mg/kg Ferrous gluconate(FG),respectively.The experimental chickens were fed and watered freely for 21 days.The results showed that there were no significant differences in average daily feed intake,average daily gain and feed to gain ratio among the three treatment groups(P>0.05).But,the diarrhea rate of Yellow-feathered chickens in 1200 mg/kg and 2400 mg/kg ferrous gluconate groups was significantly higher than that in control group(P<0.05).The activities of plasma induced nitric oxide synthase and creatine kinase and the content of malondialdehyde in jejunum mucosa of 2400 mg/kg FG group were significantly higher than those of the control group(P<0.05).The gene expressions of nuclear factor E2-related factor 2,caudal homologous transcription factor 2 and closed small cycloprotein-1 in jejunum tissues of 1200 mg/kg and 2400 mg/kg FG groups were significantly lower than those of control group(P<0.05).In conclusion,dietary supplementation of high dose of ferrous gluconate(1200 mg/kg to 2400 mg/kg)has no negative effect on growth performance of Yellow-feathered chickens aged 22 to 42 days,but it induces oxidative damage of jejunum mucosa of chickens,and the degree of damage is related to the dosage of ferrous gluconate,which in turn led to a significant increase in diarrhea rates.

关 键 词：铁 黄羽肉鸡 空肠黏膜 氧化应激 

分 类 号：S816.71[农业科学—饲料科学]