猪瘟病毒抗体检测技术研究进展  

Research Progress on Antibody Detection Technology associated with Classical Swine Fever Virus

作　　者：颜士琦 李复坤 王红彩 周晓敏 樊全宝 冯钰鑫 齐冬梅 赖月辉 黄福强 YAN Shiqi;LI Fukun;WANG Hongcai;ZHOU Xiaomin;FAN Quanbao;FENG Yuxin;QI Dongmei;LAI Yuehui;HUANG Fuqiang(School of Animal Science and Technology,Foshan University,Foshan Guangdong 528231;Guangdong Winsun Bio-Phamaceutical Co.,Ltd,Guangzhou Guangdong 511356)

机构地区：[1]佛山大学动物科技学院,广东佛山528231 [2]广东永顺生物制药股份有限公司,广东广州511356

出　　处：《广东畜牧兽医科技》2024年第6期80-85,共6页Guangdong Journal of Animal and Veterinary Science

摘　　要：猪瘟(Classical swine fever,CSF)是由猪瘟病毒(Classical swine fever virus,CSFV)引起的猪的一种高度传染性和致死性的病毒性疾病。该病流行广泛,呈世界性分布;发病率高、死亡率高,严重危害养猪业。目前疫苗免疫接种仍然是全球大多数养猪国家防控CSF的主要手段,因此正确利用试验室或临床检测技术检测CSFV抗体,准确评价猪群CSF疫苗免疫效果和评估非免疫猪群中是否存在CSFV感染,并在此基础上提出CSF防控措施至关重要。该文针对正相间接血凝试验、病毒中和试验、免疫层析技术、酶联免疫吸附试验、蛋白质芯片技术、荧光微球技术等目前常用的CSFV抗体检测技术和方法进行综述,以期为CSF免疫评估和CSF综合防控提供理论参考。Classical swine fever(CSF)is a highly contagious and fatal viral disease of pigs caused by Classical swine fever virus(CSFV).CSF is widespread and distributed worldwide.The high morbidity and mortality rate seriously harm the pig industry.At present,vaccine immunization is still the main means for the prevention and control of CSF in most of pig farming countries in the world.Therefore,it is very important to correctly detect CSFV antibody by laboratory or clinical detection technology,accurately evaluate the immune efficiency of CSFV vaccine in pigs and evaluate whether CSFV infection exists in non-immune pigs,and then propose CSF prevention and control measures.In this paper,we reviewed the common detection techniques and methods of CSFV antibody,such as Indirect hemagglutination test(IHA),Virus neutralization test in vitro(VNT),Immunochromatography assay(ICA),Enzyme-linked immunosorptive test(ELISA),Protein chip technology,Bead-based multiplex assay(BBMA),in order to provide theoretical reference for immune evaluation and comprehensive prevention and control of CSF.

关 键 词：猪瘟病毒 抗体 检测技术 

分 类 号：S852.651[农业科学—基础兽医学]