近红外光谱结合HPLC对清肺抑火片多指标成分的含量测定  

Determination of Multiple Bioactive Constituents of Qingfei Yihuo Tablets by NIRS and HPLC

作　　者：周燕 张纯 陈叶青 ZHOU Yan;ZHANG Chun;CHEN Ye-qing(Jiaxing Institute for Food,Drug and Product Quality Control,Jiaxing 314001,China)

机构地区：[1]嘉兴市食品药品与产品质量检验检测研究院,浙江嘉兴314050

出　　处：《食品与药品》2024年第6期506-509,共4页Food and Drug

基　　金：嘉兴市科技计划(应用性基础研究)项目(编号:2021AY10078)。

摘　　要：目的创建一种能快速测定清肺抑火片中黄芩苷、大黄酚、栀子苷、芒果苷、盐酸小檗碱、白花前胡甲素6种成分含量的方法。方法采用高效液相色谱法(HPLC)测定清肺抑火片中黄芩苷、大黄酚、栀子苷、芒果苷、盐酸小檗碱、白花前胡甲素含量,采用偏最小二乘法(PLS)建立清肺抑火片近红外定量分析模型。结果创建的近红外定量分析模型的相关系数(R2)分别为0.9584,0.9217,0.9555,0.9512,0.9388,0.9221;均方差(RMSECV)分别为0.226,0.017,0.062,0.008,0.026,0.005。结论利用近红外光谱建立的定量模型,能对清肺抑火片中黄芩苷、大黄酚、栀子苷、芒果苷、盐酸小檗碱、白花前胡甲素的含量进行无损测定。Objective To establish a rapid method for simultaneous determination of baicalin,chrysophanol,geniposide,mangiferin,berberine hydrochloride and praeruptorin A in Qingfei Yihuo Tablets.Methods The contents of baicalin,chrysophanol,geniposide,mangiferin,berberine hydrochloride and praeruptorin A in Qingfei Yihuo Tablets were determined by HPLC.Partial least squares method(PLS)was used to establish the near infrared quantitative analysis model of baicalin,chrysophanol,geniposide,mangiferin,berberine hydrochloride and praeruptorin A in the samples.Results R2 of the established near infrared quantitative analysis models for baicalin,chrysophanol,geniposide,mangiferin,berberine hydrochloride and praeruptorin A were 0.9584,0.9217,0.9555,0.9512,0.9388 and 0.9221,respectively.RMSECV were 0.226,0.017,0.062,0.008,0.026 and 0.005,respectively.Conclusion The quantitative analysis models established by NIRS can be used for rapid and nondestructive determination of baicalin,chrysophanol,geniposide,mangiferin,berberine hydrochloride and praeruptorin A in Qingfei Yihuo Tablets.

关 键 词：清肺抑火片 近红外光谱 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]