SBSE-TD/GC-MS测定不同时期金银花挥发性香气成分  

Determination of Volatile Aroma Components of Lonicerae Japonicae Flos in Different Periods by SBSE-TD/GC-MS

作　　者：王辉 吴鸿 WANG Hui;WU Hong(Shanghai Botanical Garden,Shanghai 200231,China)

机构地区：[1]上海植物园,上海200231

出　　处：《食品与药品》2024年第6期I0007-I0011,共5页Food and Drug

基　　金：上海市卫生健康委员会中医药文化宣传教育基地项目[沪卫中发(2022)23号项目]。

摘　　要：目的分析金银花不同发育时期鲜花挥发性香气成分及含量。方法采用搅拌子吸附萃取-脱附/气相色谱质谱技术(SBSE-TD/GC-MS)定性,结合内标定量,获得各时期金银花中挥发性香气成分组成和含量。结果大白期、银花期、金花期检出香气成分的含量依次为3.35,8.42,9.88μg/g FW。大白期检出15个香气成分,香气最弱;银花期检出33个香气成分,香气较强;金花期检出40个香气成分,香气最强。大白期有较高含量的酸类,主要成分为十六酸、乙酸、苯甲酸、柠檬烯等;银花期检出主要成分为酯类、萜烯类和酸类,苯甲酸甲酯、香叶烯D、十六酸等含量较高;金花期主要成分为酯类、萜烯类和醇类,苯甲酸甲酯、香叶烯D、芳樟醇、苯甲酸叶醇酯等含量较高。结论本研究方法获得的香气成分以低碳芳香成分为主,从大白、银花到金花期成分数量增多、香气含量增大,酸类成分含量降低,萜烯类、醇类、酯类成分含量升高,可为不同时期金银花的功能开发提供依据。Objective To analyze the volatile aroma components and contents of Lonicerae japonicae Flos at different developmental stages.Methods The composition and contents of volatile aroma components in Lonicerae japonicae Flos at different stages were obtained by stir bar sorption extraction-desorption/gas chromatography-mass spectrometry(SBSE-TD/GC-MS)combined with internal standard quantification.Results The contents of aroma components detected in“Dabai”,“Yinhua”and“Jinhua”period were 3.35,8.42,9.88μg/g FW,respectively.15 aroma components were detected in“Dabai”period,and the aroma was the weakest.A total of 33 aroma components were detected in“Yinhua”period,and the aroma was strong.40 aroma components with the strongest aroma were detected in“Jinhua”period.There was a high content of acids in“Dabai”period,the main components were hexadecanoic acid,acetic acid,benzoic acid,limonene,etc.;the main components detected at“Yinhua”period were esters,terpenes and acids,and the contents of methyl benzoate,geraniol D and hexadecanoic acid were high.The main components detected at“Jinhua”period were esters,terpenes and alcohols,and the contents of methyl benzoate,geraniol D,linalool,and hecenyl benzoate were high.Conclusion The aroma components obtained by this method are mainly low carbon aroma components.From“Dabai”,“Yinhua”to“Jinhua”period,the number and contents of aroma components increase,the contents of acid components decrease,and the contents of terpenes,alcohols and esters increase,which can provide a basis for the functional development of Lonicerae japonicae Flos in different periods.

关 键 词：金银花 搅拌子吸附萃取脱附 气相色谱质谱技术 挥发性香气成分 分析 

分 类 号：R284.1[医药卫生—中药学]