甘蓝型油菜苗期赤霉素含量及其代谢关键基因转录特性  

Gibberellin metabolism level and key gene transcription characteristics at seedling stage of Brassica napus L.

作　　者：高少凡 韦丁一 何庆彪 徐劲松 张学昆 谢伶俐 许本波[1] GAO Shao-fan;WEI Ding-yi;HE Qing-biao;XU Jin-song;ZHANG Xue-kun;XIE Ling-li;XU Ben-bo(College of Life Science,Engineering Research Center of Ecology and Agricultural Use of Wetland,Ministry of Edu-cation,Yangtze University,Jingzhou 434025,Hubei,China;Hubei Lizhong Seed Industry Technology CO.,LTD,Xiantao 433000,China;College of Agriculture,Yangtze University,Jingzhou 434025,China)

机构地区：[1]长江大学湿地生态与农业利用教育部工程研究中心,生命科学学院,湖北荆州434025 [2]湖北利众种业科技有限公司,湖北仙桃433000 [3]长江大学农学院,湖北荆州434025

出　　处：《中国油料作物学报》2024年第6期1240-1250,共11页Chinese Journal of Oil Crop Sciences

基　　金：农业农村部项目(15214011);湖北省农业厅项目(鄂农油[2021]9号)。

摘　　要：为了解GA在甘蓝型油菜苗期杂种优势中的作用及温度对GA合成的影响,本研究选用杂种F_1产量优势较强的杂交组合YG2009×YC4及其亲本为试验材料,分别在5、15、25℃生长,分析杂种F_1及亲本苗龄20 d时农艺性状与GA总量的关系及GA总量随温度变化的趋势,并利用qPCR技术分析GA合成途径关键酶基因的相对转录水平。结果表明温度显著影响甘蓝型油菜幼苗中GA含量,进而影响幼苗的生长,随着温度的升高,不同材料株高、叶片数、叶宽、叶长、鲜重、干重等表型性状呈显著上升趋势;典型相关分析发现,表型性状与GA总量呈正相关,即GA总量升高,有利于甘蓝型油菜幼苗的生长。YG2009×YC4植株中GA总量随温度的升高呈上升趋势,25℃时达到最高,YG2009×YC4植株中GA总量比母本YG2009提高83.01%,比父本YC4提高100.72%,达到差异显著水平。25℃时,转录水平分析发现甘蓝型油菜不同部位GA代谢关键酶基因存在差异转录,导致各部位GA总量存在较大差异。灰色关联分析发现,GA2ox6相对转录水平与根中GA总量灰色关联度最高,GID1相对转录水平与茎中GA总量灰色关联度最高,GA20ox4相对转录水平与叶中GA总量灰色关联度最高,推测GA2ox6、GID1、GA20ox4相对转录水平对植株中GA总量影响较大。In order to understand the role of GA in heterosis of Brassica napus at seeding stage and effect of temperature on GA synthesis.In this study,the hybrid combination YG2009×YC4 with strong yield advantage of hybrid F1 and its parents YG2009 and YC4 were selected as experimental materials,which grew at 5,15 and 25℃,respectively.The relationship between agronomic traits and total GA content of hybrid F1 and parents at 20 days of seedling age and the trend of total GA content with temperature were analyzed,and the relative transcription level of key enzyme genes in GA synthesis pathway was analyzed by qPCR.The results showed that temperature significant⁃ly affected the GA content in B.napus seedlings,which in turn affected the growth of seedlings.With the increase of temperature,the phenotypic traits such as plant height,leaf number,leaf width,leaf length,fresh weight and dry weight of different materials showed a significant upward trend.The total GA content in plants changed significantly with temperature.Canonical correlation analysis showed that phenotypic traits were positively correlated with the to⁃tal GA content,that is,the total GA content increased,which was beneficial to the growth of B.napus seedlings.The total GA content in YG2009×YC4 plants increased with the increase of temperature,and reached the highest at 25℃.The total GA content in YG2009×YC4 plants was 83.01%higher than that of female parent YG2009 and 100.72%higher than that of male parent YC4,reaching a significant difference level.At 25℃,the relative tran⁃scription level analysis of key enzyme genes of GA metabolism in different parts showed that there were different transcriptions of key enzyme genes in different parts of B.napus,resulting in a large difference in the total GA con⁃tent in each part.Grey correlation analysis showed that the relative transcription level of GA2ox6 had the highest grey correlation degree with the total GA content in roots,the relative transcription level of GID1 had the highest grey correlation de

关 键 词：温度 甘蓝型油菜 赤霉素 基因转录水平 表型特征 

分 类 号：S565.4[农业科学—作物学]