大豆重要性状功能基因KASP标记的开发与验证  

Development and validation of KASP markers for functional genes of important traits in soybean

作　　者：张君权 武婷婷[2] 姚翔宇 熊果 蒋炳军[2] 陈庆山[1] 孙石[2] 韩天富[1,2] ZHANG Jun-quan;WU Ting-ting;YAO Xiang-yu;XIONG Guo;JIANG Bing-jun;CHEN Qing-shan;SUN Shi;HAN Tian-fu(College of Agriculture,NE China Agricultural University,Harbin 150006,China;Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]东北农业大学农学院,黑龙江哈尔滨150006 [2]中国农业科学院作物科学研究所,北京100081

出　　处：《中国油料作物学报》2024年第6期1251-1267,共17页Chinese Journal of Oil Crop Sciences

基　　金：国家现代农业产业技术体系建设专项(CARS-04)。

摘　　要：为实现大豆高通量基因分型和分子标记辅助选择(MAS),根据大豆中已报道的重要性状基因功能位点,开发了22个KASP标记,分别对应大豆结荚习性(Dt1基因)、多粒荚(Ln基因)、炸荚(Pdh1基因)、籽粒大小(GmSSS1、GmST05基因)、开花期(J,GmPRR3a,GmPRR3b,GmFUL2a,GmLHY1a,GmLHY1b,GmSOC1a,E4基因)、蛋白和油分含量(GmSWEET39基因)、胡萝卜素含量(GmCCD4基因)、磷利用效率(GmPHF1基因)、氮利用效率(GmGLP20.4基因)、根瘤共生兼容性(Rj2基因)和细胞分裂素合成(GmCXK7-1基因)等性状和基因。对标记的分型鉴定表明,除e4-sore大片段标记不能区分杂合样本外,其余21个标记均能对杂合和两种纯合基因型进行准确分型。结合表型信息对多粒荚、叶形和结荚习性标记的表型选择效率进行了评估,结果表明多粒荚Ln基因分子标记对表型的选择效率为84.0%,对叶形的选择效率为94.8%,结荚习性Dt1基因分子标记对表型的选择效率为87.4%,显示出较高的预测准确性。本实验开发的22个功能标记可对基因型进行准确分型,在大豆分子标记辅助选择中具有重要利用价值。Functional marker(FM)is one of the most useful molecular markers in crop breeding,which facilitates the realization of high-throughput genotyping and marker-assisted selection(MAS).In this study,22 KASP markers were developed according to the reported functional loci of important traits in soybean,including stem growth habit(Dt1),multi-seed pods(Ln),shattering pods(Pdh1),seed size(GmSSS1,GmST05),flowering time(J,GmPRR3a,GmPRR3b,GmFUL2a,GmLHY1a,GmLHY1b,GmSOC1a,E4),protein and oil content(GmSWEET39),carotene content(GmCCD4),phosphorus use efficiency(GmPHF1),nitrogen use efficiency(GmGLP20.4),symbiotic compatibility in nodulation(Rj2)and cytokinin biosynthesis(GmCXK7-1).All of the 21 markers could be used to accurately classify heterozygous and homozygous genotypes,except that the marker of e4-sore could not distinguish heterozygous samples.Combining with phenotypic information,the selection efficiency of multi-seed pod,leaf shape and stem growth habit markers was analyzed.The results showed that the selection efficiency of multi-seed pod(Ln)markers on phenotype was 84.0%,that on leaf shape was 94.8%,and that of stem growth habit(Dt1)markers on phenotype was 87.4%,showing a high level of selection accuracy.The 22 functional markers developed in this study can be used to accurately identify the genotypes and have important utilization value in marker assisted breeding of soybean.

关 键 词：大豆 功能标记 KASP 分子标记辅助选择 

分 类 号：S565.1[农业科学—作物学]