油茶根腐病内生拮抗促生细菌筛选及诱导系统抗性研究  

Screening and systematic resistance induction of endogenous antagonistic growth-promoting bacteria against Camellia oleifera root rot

作　　者：李丽丽 曾广宇 王继春 何苑皞[1] LI Li-li;ZENG Guang-yu;WANG Ji-chun;HE Yuan-hao(Central South University of Forestry and Technology,Key Laboratory of National Forestry and Grassland Admin-istration on Control of Artiffcial Forest Diseases and Pests in South China/Hunan Provincial Key Laboratory for Con-trol of Forest Diseases and Pests/Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees,Changsha 410004,China;Guangxi Forestry Science Research Institute,Nanning 530002,China;State-owned Dagui Mountain Forest Farm in Guangxi Zhuang Autonomous Region,Hezhou 542800,China)

机构地区：[1]中南林业科技大学,南方人工林病虫害防控国家林草局重点实验室/森林有害生物防控湖南省重点实验室/经济林培育与保护教育部重点实验室,湖南长沙410004 [2]广西壮族自治区林业科学院,广西南宁530002 [3]广西壮族自治区国有大桂山林场,广西贺州542800

出　　处：《中国油料作物学报》2024年第6期1390-1397,共8页Chinese Journal of Oil Crop Sciences

基　　金：国家自然科学基金(31600515);广西科技重大专项(桂科AA20302021-3);湖南省自然科学基金青年基金(2019JJ50999)。

摘　　要：为丰富油茶根腐病生防资源并探究其抗病机理,采用平板对峙法和比色法等从油茶根系内生菌中筛选出拮抗促生细菌,并测定其对油茶根系防御酶活性及防御基因的影响。结果表明:结合拮抗效果、产IAA、溶有机磷、无机磷及产铁载体能力,从油茶根系筛选到两株高效内生拮抗促生菌AF9和AH6,经鉴定均为芽孢杆菌;二者均可以诱导油茶根系防御酶活性提高,超氧化物歧化酶(SOD)、苯丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)以及过氧化物酶(POD)活性分别在8 h、10 h、12 h和24 h达到峰值;PAL、RBOHC和POD基因分别在处理10 h、12 h及24 h时高度表达,对照组基因无明显表达。表明拮抗促生菌AF9和AH6可以诱导油茶产生系统抗性,为后期菌剂的研制以及油茶根腐病的生物防治奠定基础。In order to enrich the biocontrol resources of Camellia oleifera root rot disease and explore its resis⁃tance mechanism,antagonistic growth-promoting bacteria were screened from endophytic bacteria of C.oleifera root by plate confrontation method and colorimetric method,and their effects on defense enzyme activities and defense genes of C.oleifera root were measured.The results showed that two strains of efficient endophytic growth promoters AF9 and AH6 were screened from the root system of C.oleifera by combining the antagonistic effect,IAA produc⁃tion,organic phosphorus soluble,inorganic phosphorus production and iron-producing carrier capacity.Both strains were identified as Bacillus.Both of them could increase the root defense enzyme activities,and the activities of su⁃peroxide dismutase(SOD),phenylalanine ammoniase(PAL),polyphenol oxidase(PPO),and peroxidase(POD)reached the peak at 8 h,10 h,12 h,and 24 h,respectively.PAL,RBOHC and POD,genes were highly expressed at 10 h,12 h,and 24 h,respectively,while no significant expression was found in control group.The results indicated that the antagonistic growth promoting bacteria AF9 and AH6 could induce systemic resistance of C.oleifera,which laid the foundation for the development of later bacteriotics and biological control of C.oleifera root rot.

关 键 词：内生菌 油茶 诱导抗性 防御酶活性 

分 类 号：S763.1[农业科学—森林保护学]