基于UHPLC-QE-MS方法结合网络药理学探讨炆何首乌抗衰老的作用机制  

Study on the Anti-aging Mechanism of Stewed Polygoni Multiflori Based on UHPLC-QE-MS and Network Pharmacology

作　　者：黎红 王昆 陈瑶[2] 曾庆财 周智文 LI Hong;WANG Kun;CHEN Yao;ZENG Qingcai;ZHOU Zhiwen(Pharmacy Department,Affiliated Hospital of Jiangxi College of Traditional Chinese Medicine/Fuzhou Hospital of Traditional Chinese Medicine Hospital,Jiangxi Province,Fuzhou 344000,China;School of Pharmacy,Jiangxi University of Chinese Medicine,Jiangxi Province,Nanchang 330004,China)

机构地区：[1]江西中医药高等专科学校附属医院/抚州市中医医院药剂科,江西抚州344000 [2]江西中医药大学药学院,江西南昌330004

出　　处：《中国中医药现代远程教育》2025年第2期100-103,共4页Chinese Medicine Modern Distance Education of China

基　　金：江西省中医药管理局科技课题【No.2023B1387】。

摘　　要：目的探讨炆何首乌抗衰老的作用机制。方法通过超高效液相色谱-质谱联用(UHPLC-QE-MS)技术,鉴定生、炆何首乌活性成分;利用相关数据库筛选出炆何首乌的主要活性成分及作用靶点,并借助注释、可视化和综合发现数据库(DAVID)开展京都基因与基因组百科全书(KEGG)富集分析以及基因本体论(GO)功能分析。结果共得到炆何首乌有效成分82个,其中抗衰老相关疾病靶点共40个;得到丝氨酸/苏氨酸激酶1(AKT1)、表皮生长因子受体(EGFR)、缺氧诱导因子1亚基α(HIF1A)3个核心靶点,主要富集在癌症的蛋白聚糖、癌症的形成途径等信号通路上。结论推测炆何首乌主要通过细胞凋亡过程的负调控、基因表达的负调控等途径,发挥抗衰老作用。Objective To explore the anti-aging mechanism of stewed Polygonum multiflori.Methods Identifying the active ingredients of raw and fermented Polygonum multiflorum using UHPLC-QE-MS technology,screening out the main active ingredients and targets of fermented Polygonum multiflorum using relevant databases,and KEGG enrichment analysis and GO functional analysis were con-ducted using DAVID database.Results A total of 82 effective ingredients were obtained from Polygonum multiflorum,and a total of 40 targets were identified for the treatment of age-related diseases.Obtaining three core targets:AKT1,EGFR,and HIF1A,which are mainly enriched in proteoglycans in cancer and signaling pathways related to cancer formation.Conclusion Stewed Polygonum multiflorum mainly exerts anti-aging effects through negative regulation of cell apoptosis process and negative regulation of gene expression.

关 键 词：网络药理学 炆何首乌 抗衰老 UHPLC-QE-MS 

分 类 号：R285[医药卫生—中药学]