不同圈养生境下马鹿肠道菌群结构的比较  

Comparison of intestinal flora structure of red deers in different captive conditions

作　　者：史春苗 李伟[2] 仉伟[2] 郜振杰 张永宾 吴弘 赵大鹏[1] SHI Chunmiao;LI Wei;ZHANG Wei;GAO Zhenjie;ZHANG Yongbin;WU Hong;ZHAO Dapeng(School of Life Sciences,Tianjin Normal University,Tianjin 300387,China;Jinan Zoo,Jinan 250031,China;Shijiazhuang Zoo,Shijiazhuang 050200,China)

机构地区：[1]天津师范大学生命科学学院,天津300387 [2]济南动物园,济南250031 [3]石家庄动物园,石家庄050200

出　　处：《天津师范大学学报（自然科学版）》2024年第6期20-28,共9页Journal of Tianjin Normal University：Natural Science Edition

基　　金：中央财政林业补助资金项目-野生动物监测专项(53H20004);生态环境部生物多样性调查、观测与评估项目(2019-01);国家自然科学基金资助项目(31772468).

摘　　要：为了解国家二级重点保护物种马鹿(Cervus elaphus)肠道菌群的结构特征及其影响因素,以济南动物园和石家庄动物园圈养生境下的马鹿种群为样本组,基于非损伤采样技术,应用16S rRNA高通量测序技术和生物信息学分析,比较2个样本组的肠道菌群结构特征.结果显示:①马鹿2个样本组的共有优势菌门包括厚壁菌门(Firmicutes)、拟杆菌门(Bacteroidota)、螺旋体门(Spirochaetota)和髌骨细菌门(Patescibacteria),共有优势菌属包括UCG-005属、Rikenellaceae_RC9_gut_group属和Christensenellaceae_R-7_group属;②济南样本组中厚壁菌门和纤维杆菌门的丰度显著高于石家庄样本组的丰度,石家庄样本组中UCG-005属丰度显著高于济南样本组的丰度;③基于Alpha和Beta多样性分析,2个样本组间肠道菌群结构存在显著差异,济南样本组的多样性显著高于石家庄样本组的多样性.基于以上研究结果,初步推测样本组间肠道菌群存在差异主要是食物组成不同所致.In order to learn the structural characteristics and influencing factors of intestinal flora of red deer(Cervus ela-phus),which is a national secondary key protected species,the captive populations in Jinan Zoo and Shijiazhuang Zoo were chosen as focus subjects.Non-invasive sampling technology was used,and 16S rRNA high-throughput sequencing technology and bioinformatics analysis were proceeded to make comparison on intestinal flora characteristics between these two sample groups.The results showed that:①Common dominant bacterial phyla of two sample groups included Firmicutes,Bacteroidota,Spirochaetota,and Patescibacteria,while their common dominant genera included UCG-005,Rikenellaceae_RC9_gut_group,and Christensenellaceae_R-7_group;②The abundance of Firmicutes and Fibrinobacteria in Jinan sample group was significa-ntly higher than that in Shijiazhuang sample group.The abundance of UCG-005 in Shijiazhuang sample group was significantly higher than that in Jinan sample group;③Significant intergroup differences were found in intestinal flora structure based on Alpha and Beta diversity analysis.The diversity of Jinan sample group was significantly higher than that of Shijiazhuang sam-ple group.Based on the above findings,it is preliminarily assumed that such intergroup variations in intestinal flora are mainly due to differences in food composition.

关 键 词：马鹿 肠道菌群 多样性 食物结构 高通量测序 生物信息学 

分 类 号：Q958[生物学—动物学]