响应面法优化山楂皮渣总黄酮提取工艺及抗氧化活性研究  

Optimization Ethanol Extraction of Total Flavonoids from Hawthorn Pomace by Response Surface Methodology and Antioxidant Activity

作　　者：张春丹[1,2,3] 乔洁 解春艳[1,2,3] 林琳 武张飞[1,2,3] 尚校兰 Zhang Chundan;Qiao Jie;Xie Chunyan;Lin Lin;Wu Zhangfei;Shang Xiaolan(Langfang Normal University,Langfang 065000,China;Technology Innovation Center for Utilization of Edible and Medicinal Fungi in Hebei Province,Langfang 065000,China;Langfang Key Laboratory of Food Nutrition and Safety,Langfang 065000,China)

机构地区：[1]廊坊师范学院,河北廊坊065000 [2]河北省食药用菌资源高值利用技术创新中心,河北廊坊065000 [3]廊坊市食品营养与安全重点实验室,河北廊坊065000

出　　处：《廊坊师范学院学报（自然科学版）》2024年第4期49-55,共7页Journal of Langfang Normal University(Natural Science Edition)

基　　金：廊坊市科技局自筹项目(2023013109)。

摘　　要：目的∶确定超声辅助乙醇提取山楂皮渣总黄酮工艺的最佳条件,并对其抗氧化能力进行分析评价。方法∶以山楂皮渣为原料,山楂皮渣总黄酮得率为指标,应用单因素试验分析料液比、提取时间、乙醇体积分数、超声功率对总黄酮得率的影响,并用响应面法优化总黄酮的提取工艺。结果∶料液比为1:16 g/mL,在450 W超声辅助条件下,用体积分数为70%的乙醇提取120 min为最佳工艺条件,此条件下山楂皮渣总黄酮得率可高达62.79 mg/g。结论∶超声辅助条件下,利用响应面法优化乙醇提取山楂皮渣总黄酮的工艺条件合理可靠,并得到了最佳工艺;山楂皮渣总黄酮具有很强的抗氧化能力。Objective:To optimize ultrasound-assisted ethanol extraction of the total flavonoids from hawthorn pomace and determine the antioxidant capacity of the total flavonoids.Method:Hawthorn pomace is used as the raw material,and the yield of total flavonoids from hawthorn pomace as indicator,single-factor experiments are applied to analyze the effects of solid-liquid ratio,extraction time,ethanol volume fraction,and ultrasonic power on the yield of total flavonoids.The response surface methodology is applied to optimize the extraction process of total flavonoids from hawthorn pomace.Result:The optimal extraction conditions for ultrasound-assisted ethanol extraction of total flavonoids from hawthorn pomace:with a solidliquid ratio of 1:16 g/mL,under 450 W ultrasonic power,using 70%ethanol for extraction over 120 minutes.Under these conditions,the yield of total flavonoids from hawthorn pomace can reach 62.79 mg/g.Conclusion:Optimization of ultrasonicassisted ethanol extraction of total flavonoids from hawthorn pomace by response surface methodology is reasonable and reliable,and the best optimization of extraction technology is obtained.The total flavonoids from hawthorn pomace have strong antioxidant capacity.

关 键 词：山楂皮渣 总黄酮 响应面法 超声提取 抗氧化 

分 类 号：R284.2[医药卫生—中药学]