高抗菌性光催化材料的制备及LED光驱动其抗菌性能研究  

Preparation of Highly Antimicrobial Composites and Study of Photocatalytic Antimicrobial Properties Driven by LED Light

作　　者：康健 石梓煊 李景梅[1] Kang Jian;Shi Zixuan;Li Jingmei(School of Life Sciences and Technology,Changchun University of Science and Technology,Changchun 130012,China)

机构地区：[1]长春理工大学生命科学技术学院,长春130012

出　　处：《化学学报》2024年第9期962-970,共9页Acta Chimica Sinica

基　　金：吉林省发展改革项目(2022C039-5);吉林省教育厅科学技术研究项目(JJKH20231430KJ)资助.

摘　　要：本工作采用简易的水热合成法合成了一种具有高抗菌性能的Mn-ZnO复合无机光催化材料.通过X射线衍射、扫描电子显微镜、X射线能谱、紫外-可见漫反射吸收光谱等方法对其进行了表征,证实了材料的成功制备同时表明Mn的引入提高了电子-空穴对的分离效率;光催化抗菌实验结果表明锰掺杂量为质量分数1%的Mn-ZnO复合材料对细菌的光催化抗菌性能显著高于单一的ZnO纳米材料.在LED光的驱动下,Mn-ZnO复合材料的浓度仅需125 mg/L时,其对大肠杆菌(E.coli)、金黄色葡萄球菌(S.aureus)和枯草芽孢杆菌(Bacillus subtilis)的抗菌效率在较短时间内即可分别达到98.33%、100%和100%.这说明Mn-ZnO复合光催化材料对细菌的杀伤效果十分高效且具有广谱抗菌性,只需要很短的时间且极低的浓度就可以将多种病原微生物快速杀灭.而3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐法(MTT)实验证明所合成的这种高抗菌性复合光催化材料在实验浓度下具有良好的生物相容性.本研究提供了一种廉价、绿色、简单易制备的方法合成了高抗菌性能的光催化材料,该材料也有望投入到污水处理当中,在实际抗菌领域拥有广阔的应用前景.In this study,a Mn-ZnO composite inorganic photocatalytic material with high antimicrobial properties was successfully synthesised using a facile hydrothermal synthesis method.The material was characterised by X-ray diffraction,scanning electron microscopy,energy dispersive X-ray spectra and ultraviolet-visible diffuse reflection spectra,which confirmed the successful preparation of the material and showed that the introduction of Mn improved the separation efficiency of electron-hole pairs.In order to determine the optimum molar ratio of composites selected for subsequent experiments,this experiment was carried out by comparing the photocatalytic efficiencies of Mn-ZnO composite photocatalytic materials with doping molar ratios of 1%,3%and 5%.The results demonstrated that the photocatalytic antimicrobial performance of the composites against bacteria was significantly higher than that of single ZnO nanomaterials at 1%Mn doping.In subsequent studies,a 35 W LED light was employed as the excitation light source,and the gradient dilution method and plate colony counting method were used to evaluate the photocatalytic antimicrobial performance of Mn-ZnO composite photocatalytic materials at different concentration gradients(125 mg/L,250 mg/L,375 mg/L,500 mg/L,1000 mg/L)against three bacterial species,namely,E.coli,S.aureus and Bacillus subtilis.In comparison to the pure ZnO control group,the composites demonstrated antimicrobial effects of 98.33%,100%and 100%respectively,in a shorter period of time under light conditions with only 125 mg/L.These results indicate that the Mn-ZnO composite photocatalytic materials were more effective against E.coli,S.aureus and Bacillus subtilis than the pure ZnO.This suggests that the Mn-ZnO composite photocatalytic material exhibits high efficiency and broad-spectrum antimicrobial properties,capable of rapidly destroying a multitude of pathogenic microorganisms at a low concentration.Furthermore,3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)experiments demonstrated

关 键 词：锰掺杂 氧化锌 LED光 光催化 抗菌 复合材料 

分 类 号：O643.36[理学—物理化学] O644.1[理学—化学]