电针通过促进突触再生改善脑缺血再灌注损伤大鼠的学习记忆功能  

Electroacupuncture improves learning and memory function and promotes hippocampal synaptic regeneration in rats with cerebral ischemia-reperfusion injury

作　　者：林如辉[1,2] 夏金言 马小涵 李钻芳 LIN Ruhui;XIA Jinyan;MA Xiaohan;LI Zuanfang(Innovation and Transformation Center,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China;Academy of Integrative Medicine,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China)

机构地区：[1]福建中医药大学科技创新与转化中心,福建福州350122 [2]福建中医药大学中西医结合研究院,福建福州350122

出　　处：《南方医科大学学报》2024年第12期2317-2326,共10页Journal of Southern Medical University

基　　金：国家自然科学基金(81774386);福建省自然科学基金(2023J01873,2022J01878)。

摘　　要：目的探讨电针百会、神庭对脑缺血再灌注损伤大鼠的神经保护作用机制。方法将雄性SD大鼠48只随机分为假手术组、模型组、电针组和非穴组,12只/组。采用Longa法制备局灶性缺血损伤大鼠模型。电针干预大鼠百会、神庭穴7d。观察大鼠神经功能缺损评分、脑梗死体积变化、学习记忆功能、海马CA1区病理变化、神经元及突触超微结构,并计数突触密度、血清血清γ-氨基丁酸(GABA)、海马组织中GABAARα1、钙调蛋白依赖性蛋白激酶Ⅱ(CaMKⅡ)、突触素1(SYN1)和突触后致密蛋白-95(PSD-95)蛋白及mRNA表达情况、SYN1和PSD-95表达水平。结果与模型组比较,电针组大鼠神经功能缺损评分降低(1.75±0.45 vs1.50±0.67,P<0.05);脑梗死体积降低(11.25±6.22 vs 3.51±2.21,P<0.05);逃避潜伏期降低下降(62.65±5.12 vs 51.83±6.19,P<0.05)及穿越平台次数增加(1.17±0.75 vs 3.17±0.75,P<0.05);缺血侧海马CA1区神经细胞数量增加;突触间隙宽度相对变小,突触小体相对增加(2.00±0.11 vs 4.00±0.25,P<0.05);血清中GABA含量上升(3.90±0.75vs 5.54±0.35,P<0.05)海马组织中GABAARα1、SYN1和PSD-95 mRNA表达水平升高(P<0.05);CaMKⅡmRNA表达水平下降(P<0.05)。结论电针百会、神庭可改善脑缺血再灌注损伤大鼠学习记忆功能,其机制可能通过促进突触再生,上调GABAARα1、SYN1和PSD-95表达,下调CaMKⅡ表达有关。Objective To explore the neuroprotective mechanism of electroacupuncture at the acupoints Baihui and Shenting in rats with cerebral ischemia-reperfusion(IR)injury.Methods Forty-eight male SD rats were equally randomized into sham operation group,cerebral IR model group,acupoint electroacupuncture group and non-acupoint acupuncture group.In the latter 3 groups,cerebral focal ischemic injury was induced using the Longa method;in the two electroacupuncture groups,electroacupuncture was performed either at the acupoints Baihui and Shenting or at non-acupoint sites for 7 days.The changes in neurological deficit scores,cerebral infarction volume,learning and memory function,pathologies in hippocampal CA1 area,neuronal and synaptic ultrastructures,and synaptic density of the rats were observed,and serum GABA level and mRNA and protein expressions of GABAARα1,CaMK II,SYN1 and PSD-95 in the hippocampal tissue were detected.Results Compared with those in cerebral IR model group,the rats receiving electroacupuncture at the acupoints,but not those with electroacupuncture at the non-acupoints,showed significantly decreased neurological deficit scores and cerebral infarction volume with shortened escape latency and increased platform crossings.Electroacupuncture at the acupoints significantly increased neuronal cell number,decreased the width of the synaptic gaps and increased density of synaptic bodies in the ischemic hippocampal CA1 area,resulting also in increased serum GABA levels and hippocampal expressions of GABAARα1,SYN1 and PSD-95 and lowered expression level of CaMK II.Conclusion Electroacupuncture at Baihui and Shenting improves learning and memory function of rats with cerebral IR injury possibly through a mechanism that promotes synaptic regeneration,upregulates hippocampal expressions of GABAARα1,SYN1 and PSD-95 and downregulates the expression of CaMK II.

关 键 词：脑中动脉缺血再灌注损伤 电针 学习记忆功能 突触再生 突触可塑性 

分 类 号：R245[医药卫生—针灸推拿学]