柠檬醛型黄樟组培苗微扦插研究  

Study on Micro-cuttage Propagation of Cinnamomum Porrectum ct.Citral

作　　者：丁颖 顾祎昕 魏希 李凤[1] 肖祖飞 DING Ying;GU Yixin;WEI Xi;LI Feng;XIAO Zufei(Nanchang Institute of Technology,Jiangxi Porvincial Engineering Reseach Center of Seed-breeding and Utilization of Camphor Trees,330099,Nanchang,PRC)

机构地区：[1]南昌工程学院,江西省樟树繁育与开发利用工程研究中心,南昌330099

出　　处：《江西科学》2024年第6期1148-1153,共6页Jiangxi Science

基　　金：江西省林业局樟树研究专项项目(202007)。

摘　　要：为简化组培苗育苗程序以实现黄樟良种的规模化生产,以柠檬醛型黄樟组培苗为材料,研究炼苗时间、IBA浓度、扦插基质对柠檬醛型黄樟微扦插生根的影响。结果表明,柠檬醛型黄樟组培苗微扦插生根率随炼苗时间的增加而升高,瓶苗炼苗11 d后进行微扦插,生根率达到90.91%。柠檬醛型黄樟组培苗微扦插生根率随IBA浓度的增加而升高,4 000 mg/L IBA处理生根率最高(83.33%)、根数最多、根长和根粗最大,且微扦插14 d后,组培苗基部出现不定根,与常规扦插比较,生根速度加快,生根率显著提高。蛭石和泥炭混合基质对柠檬醛型黄樟组培苗微扦插生根有显著促进作用,其中蛭石和泥炭配比为2∶1是柠檬醛型黄樟组培苗微扦插最适扦插基质,生根率最高和根数最多,生根率达86.25%。研究获得了柠檬醛型黄樟组培苗微扦插的最适条件,可为黄樟良种繁育提供参考。To simplify the seedling raising procedure of plantlets and realize the large-scale production of fine varieties of C.porrectum,the study focuses on the effects of acclimatization time,IBA concentration and cutting medium on rooting of C.porrectum ct.citral,taking tissue culture plantlets of Cinnamomum porrectum ct.citral as materials.The results show that the rooting rate of micro-cuttage of C.porrectum ct.citral increased with the longer acclimatization time.When micro-cuttage was performed after 11 days of acclimatization,the rooting rate reached 90.91%.The rooting rate of micro-cuttage of C.porrectum ct.citral increased with the increase of IBA concentration.The highest rooting rate(83.33%),greatest number of root number,the largest root length and thickness were observed when the concentration of IBA reaches 4000 mg/L.Additionally,adventitious roots appeared at the base of the tissue culture seedlings 14 days after micrio-cuttage,showing a faster rooting speed and improved rooting rate compared to conventional cuttings.The mixture medium of vermiculite and peat significantly improved the rooting of the micro-cuttage of C. porrectum ct. citral, with a 21 ratio of vermiculite to peat being the optimal substrate,resulting in the highest rooting rate of 86.25% and the greatest number of roots. The studyidentifies the optimum conditions for micro-cutting of C. porrectum ct. citral tissue plantletswere obtained, which could provide reference for the well-bred breeding of C. porrectum.

关 键 词：柠檬醛型黄樟 组培苗 微扦插 

分 类 号：S792.23[农业科学—林木遗传育种]