La元素对脐橙柠檬酸代谢相关基因表达的影响  

The Effect of La Element on the Expression of Genes Related to Citric Acid Metabolism in Navel Orange

作　　者：公旭晨 王小玲[1] 毛积鹏 姚东良 高柱[1,2] GONG Xuchen;WANG Xiaoling;MAO Jipeng;YAO Dongliang;GAO Zhu(Institute of Biological Resources,Jiangxi Academy of Sciences/Jiangxi Kiwifruit Engineering Research Center,330096,Nanchang,PRC;Jinggangshan Biotechnology Research Institute,Jiangxi Academy of Sciences,343016,Ji’an,Jiangxi,PRC)

机构地区：[1]江西省科学院生物资源研究所,江西省猕猴桃工程研究中心,南昌330096 [2]江西省科学院井冈山分院,江西吉安343016

出　　处：《江西科学》2024年第6期1157-1163,共7页Jiangxi Science

基　　金：国家自然科学基金项目(31760551)。

摘　　要：为探究影响脐橙柠檬酸积累的关键轻稀土元素,并筛选参与轻稀土元素影响柠檬酸积累过程的相关基因,选取‘纽荷尔’脐橙为主要试材,对其果实发育过程中轻稀土元素含量、柠檬酸含量进行测定;同时使用不同浓度轻稀土元素溶液外源处理‘纽荷尔’脐橙果实与叶片进行转录组测序,并对测序结果进行分析,筛选相关基因。结果表明,‘纽荷尔’脐橙发育过程中La元素,是影响果实柠檬酸积累的主要元素;外施硝酸镧处理脐橙叶片与果实后,通过转录组分析得到2768个差异表达基因,其中筛选到5个参与柠檬酸代谢过程且处理前后果实中表达水平存在差异的基因:不同浓度轻稀土元素溶液处理后果实中CitPEPC1、CitPEPC2表达上调,CitPEPCK1、CitIDH3、CitGS3表达下调。综上所述,La元素在果实中通过正调控柠檬酸降解相关基因表达、负调控柠檬酸合成相关基因表达,进而抑制果实发育过程中柠檬酸的积累。In order to explore the key light rare earth elements affecting citric acid accumulation in navel orange(Citrus sinensis(L.)Osbeck),and screen the related genes involved in the process of light rare earth elements affecting citric acid accumulation,‘Newhall’Navel Oranges were selected as the primary test material to measure the content of light rare earth elements and citric acid during fruit development;Additionally,transcriptome sequencing was performed on‘Newhall’navel orange fruits and leaves treated with exogenous light rare earth element solutions of varying concentrations,and sequencing results were analyzed to screen for relevant genes.The results showed that the content of La was significantly correlated with the level of citric acid in the fruit,which was the main element affecting the accumulation of citric acid in the fruit;2768 differentially expressed genes were obtained through transcriptome analysis,among which five genes involved in citric acid metabolism showed differential expression levels before and after treatment. Aftertreatment with different concentrations of light rare earth element solutions, the expressionof CitPEPC1 was up-regulated, and the expression of CitPEPCK1,CitIDH3,and CitGS3were down regulated in fruits. In summary, the La element hinders the accumulation ofcitric acid during fruit development by positively regulating the expression of genes related tocitric acid degradation and negatively regulating the expression of genes related to citric acidsynthesis.

关 键 词：脐橙 柠檬酸 转录组 LA 轻稀土元素 

分 类 号：S666[农业科学—果树学]