杨-药复合经营林主要挥发物对光肩星天牛和美国白蛾的驱诱作用初步研究  

Attractant Effects of the Main Volatiles in the Poplar-herb Compound Management Forests on Anoplophora glabripennis and Hyphantria cunea

作　　者：代丽蕊 丁昌俊[2] 苏晓华[2] 沈应柏[3] 刘俊龙[4] 王梓豪 白心远 苗露澜 曲冠证[1] 张伟溪[2] DAI Li-rui;DING Chang-jun;SU Xiao-hua;SHEN Ying-bai;LIU Jun-long;WANG Zi-hao;BAI Xin-yuan;MIAO Lu-lan;QU Guan-zheng;ZHANG Wei-xi(National Key Laboratory of Forest Genetics and Breeding,Northeast Forestry University,Harbin 150040,Heilongjiang,China;National Key Laboratory of Forest Genetics and Breeding,Key Laboratory of Forest Cultivation of the State Forestry and Grassland Administration,Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091,China;Beijing Forestry University,Beijing 100083,China;Academy of Forestry Anhui,Hefei 230031,Anhui,China)

机构地区：[1]林木遗传育种全国重点实验室东北林业大学,黑龙江哈尔滨150040 [2]林木遗传育种全国重点实验室国家林业和草原局林木培育重点实验室中国林业科学研究院林业研究所,北京100091 [3]北京林业大学,北京100083 [4]安徽省林业科学研究院,安徽合肥230031

出　　处：《林业科学研究》2024年第6期33-44,共12页Forest Research

基　　金：“十四五”国家重点研发计划课题(2021YFD2201201)。

摘　　要：[目的]筛选杨-知母、杨-亳菊和杨-薄荷复合经营试验林中主要挥发物成分,室内测定其对光肩星天牛和美国白蛾的驱诱作用。[方法]采用动态顶空吸附法收集3种杨-药复合经营试验林内药用植物地上、整株及空气中的挥发物,利用GC-MS分析鉴定挥发物组分并筛选主要挥发物,室内测定光肩星天牛和美国白蛾对它们的EAG和嗅觉行为反应。[结果]分别从杨-知母、杨-亳菊、杨-薄荷试验林中筛选出主要挥发物乙酸叶醇酯、β-石竹烯、薄荷醇。3种化合物在测试浓度范围内均能引起光肩星天牛和美国白蛾成虫的EAG反应,且反应值随浓度升高而增强。薄荷醇对光肩星天牛成虫、美国白蛾成虫和幼虫具有明显驱避作用,且在60μg·μL^(-1)浓度对光肩星天牛雌、雄成虫驱避率最高,分别为65%和47.5%;在500μg·μL^(-1)浓度对美国白蛾雄成虫和幼虫驱避率最高,分别为60%和70%;β-石竹烯仅对2种昆虫雄虫产生引诱作用,引诱率在40%~47.5%;不同浓度乙酸叶醇酯对光肩星天牛雌、雄虫和美国白蛾雄成虫驱诱作用产生分化,表现为低浓度引诱(引诱率为45%左右),高浓度趋避(趋避率为50%左右)。对美国白蛾雌成虫具有驱避作用(52.5%),对美国白蛾幼虫具有吸引作用(45%~65%)。[结论]3种试验林中主要挥发物成分乙酸叶醇酯、β-石竹烯、薄荷醇均能引起光肩星天牛和美国白蛾的EAG和嗅觉行为反应。薄荷醇效果最为明显,对两种昆虫驱避作用显著;乙酸叶醇酯作用效果次之,主要表现为低浓度引诱,高浓度驱避作用;β-石竹烯效果最差,仅对2种昆虫雄虫产生引诱作用。研究结果可为植物源引诱剂和植物源驱避剂的开发及基于“推-拉”防治策略的绿色环保杨药复合经营模式构建提供基础理论依据。[Objective]The aims of the study are to screen the main volatile components in the experimental forests of poplar-A.asphodeloides,poplar-C.morifolium,and poplar-M.canadensis compound management,and determin their repellent or attractant effects on Anoplophora glabripennis and Hyphantria cunea indoors.[Method]The dynamic headspace adsorption method was used to collect volatile compounds from the aboveground,the whole plants of three medicinal plants,and the air in the poplar-herb compound management experimental forests.GC-MS was used to analyze and identify the volatile components,and screened the main volatile compounds.The EAG and olfactory behavioral responses of A.glabripennis and H.cunea were tested in the laboratory.[Result]The main volatile compounds,cis-3-Hexenyl acetate,beta-Caryophyllene and menthol,were screened from experimental forests of poplar-A.asphodeloides,poplar-C.morifolium,and poplar-M.canadensis,respectively.All three compounds could induce EAG responses in the tested concentration range,and the values increased with the raise of concentration.Menthol had a significant repellent effect on the adults or larvae of A.glabripennis and H.cunea,with the highest repellent rates of 65%and 47.5%on female and male A.glabripennis at 60μg·μL^(-1)concentration,while the highest repellent rates of 60%and 70%on adult and larval H.cunea at 500μg·μL^(-1)concentration,respectively.Beta-Caryophyllene could attract male insects of two species,with the attractive rate ranging from 40%to 47.5%.Different concentrations of cis-3-Hexenyl acetate induced differentiation effects on female and male A.glabripennis and adult H.cunea,with a low concentration attraction rate of about 45%,but a high concentration repellent rate of about 50%.While,it had a repellent rate of 52.5%on female adults of H.cunea and an attractive rate of 45%~65%on H.cunea larvae.[Conclusion]Cis-3-Hexenyl acetate,β-Caryophyllene and Menthol,the main volatile components in the 3 experimental forests,can both induce EAG and olfactory behaviora

关 键 词：杨药复合经营 光肩星天牛 美国白蛾 挥发物 GC-MS EAG 行为反应 

分 类 号：S763.3[农业科学—森林保护学]