3种杨树HSFB1基因CDS遗传多样性和系统演化分析  

Genetic Diversity and Phylogenetic Analysis of CDS of HSFB1 Gene in Three Populus Trees

作　　者：倪婉凝 惠楠 韦若冰 张建国[1,2] 王兆山[1,2] NI Wan-ning;HUI Nan;WEI Ruo-bing;ZHANG Jian-guo;WANG Zhao-shan(Research Institute of Forestry,Chinese Academy of Forestry,Key Laboratory of Silviculture of National Forestry and Grassland Administration,Beijing 100091,China;Collaborative Innovation Center of Sustainable Forestry in Southern China,Nanjing Forestry University,Nanjing 210037,China)

机构地区：[1]中国林业科学研究院林业研究所,国家林业和草原局林木培育重点实验室,北京100091 [2]南京林业大学南方现代林业协同创新中心,南京210037

出　　处：《林业科学研究》2024年第6期112-120,共9页Forest Research

基　　金：中国林业科学研究院林业研究所基本科研业务费项目(ZDRIF201902);国家自然科学基金项目(31770702)。

摘　　要：[目的]基于HSFB1基因,从CDS水平探讨银白杨、响叶杨和中国山杨不同群体的遗传多样性、系统发育关系以及群体遗传变异与适应异质环境之间的关系。[方法]提取HSFB1基因在各杨树样本中的CDS序列,并对各CDS序列分别进行遗传多样性、中性检验和单倍型分析。[结果]HSFB1基因在银白杨群体中的单倍型多样性和核苷酸多态性最低,在响叶杨和西南山杨群体中较低,在北方山杨和中部山杨群体中最高。各杨树群体中该基因的中性检验结果均不显著。CDS1系统发育树显示,外类群首先分枝为响叶杨和中国山杨,银白杨则聚于末端。CDS2单倍型分析显示,北方与中部山杨群体单倍型种类丰富,西南山杨、响叶杨和银白杨群体单倍型种类相对较少。[结论]不同杨树物种和群体在应对我国复杂多变的地理环境和气候条件的过程中体现出不同的适应和演化特征。[Objective]Based on the HSFB1 gene,this study aims to explore the genetic diversity,phylogenetic relationships,and the relationship between population genetic variation and adaptation to heterogeneous environments in different populations of Populus alba,Populus adenopoda,and Populus davidiana at the CDS level.[Method]The coding sequences of the HSFB1 gene were extracted from various Populus samples.Genetic diversity analysis,neutrality tests,and haplotype diversity analysis were performed.[Results]The HSFB1 gene exhibited the lowest haplotype diversity and nucleotide polymorphism in the P.alba population,followed by P.adenopoda and southwestern P.davidiana populations.In contrast,the highest diversity was observed in the P.davidiana populations in central and northern regions.The results of neutrality tests were not significant in any of the Populus populations.The phylogenetic tree of CDS1 showed that the outgroup firstly branched into P.adenopoda and P.davidiana,while P.alba clustered at the end.Haplotype analysis of CDS2 revealed that P.davidiana populations in the northern and central regionshad rich haplotype diversity,whereas the P.alba,P.adenopoda and southwestern P.davidiana populations had relatively fewer haplotypes.[Conclusion]Different Populus species and populations exhibit distinct adaptative and evolutionary characteristics in response to the complex and variable geographical environment and climate conditions in China.

关 键 词：银白杨 响叶杨 中国山杨 HSFB1 遗传多样性 单倍型网络 

分 类 号：S792.11[农业科学—林木遗传育种] Q941.2[农业科学—林学]