板栗、锥栗种内和种间嫁接亲和性初步研究  

Preliminary Study on Intraspecific and Interspecific Grafting Affinity of Castanea mollissima and C.henryi

作　　者：王一帆 江锡兵[1] 赖俊声 吴剑 吴聪连 胡伟云 李煜 龚榜初[1] WANG Yi-fan;JIANG Xi-bing;LAI Jun-sheng;WU Jian;WU Cong-lian;HU Wei-yun;LI Yu;GONG Bang-chu(Research Institute of Subtropical Forestry,Chinese Academy of Forestry,Hangzhou 311400,Zhejiang,China;Forestry College,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China;Qingyuan Bureau of Natural Resources and Planning,Qingyuan 323800,Zhejiang,China)

机构地区：[1]中国林业科学研究院亚热带林业研究所,浙江杭州311400 [2]福建农林大学林学院,福建福州350002 [3]庆元县自然资源和规划局,浙江庆元323800

出　　处：《林业科学研究》2024年第6期162-170,共9页Forest Research

基　　金：浙江省农业新品种选育重大科技专项(2021C02070-4);林业和草原科技成果国家级推广项目(2023133102)。

摘　　要：[目的]为掌握板栗、锥栗种内嫁接以及板栗和锥栗种间嫁接亲和性差异,筛选广亲和性砧穗组合应用于生产实践。[方法]以板栗、锥栗种内和种间4种嫁接类型1年生嫁接苗为材料,对其嫁接口愈合情况、生长性状、叶片表型及光合生理指标进行调查测定,并采用石蜡切片对嫁接口愈合情况进行细胞学观测,统计数据进行综合评价。[结果](1)4种嫁接类型中,板栗和锥栗种内嫁接成活率为35.71%~91.33%,且嫁接口愈合情况良好,而板栗和锥栗种间嫁接成活率仅为16.67%~45.00%,且存在部分嫁接口愈合不良现象;(2)板栗或锥栗种内嫁接组合苗高、地径和叶片数量性状表现均显著优于板栗和锥栗种间嫁接组合(p<0.05);(3)不同嫁接类型叶表型性状均存在差异,其中板栗种内嫁接组合叶面积和叶片厚度均显著大于板栗嫁接锥栗组合;锥栗种内嫁接组合叶柄长度显著大于锥栗嫁接板栗组合;(4)细胞学观察发现,板栗或锥栗种内嫁接砧穗间隔离层消失,砧、穗结合为一体,而种间嫁接砧穗间有隔离层出现;(5)各嫁接组合综合评价得分排名由高到低依次为A4>A7>A3>A2>A1>A8>A5>A6。[结论]板栗和锥栗种间嫁接存在一定的亲和性,但普遍低于板栗或锥栗种内嫁接组合的亲和性。[Objective]To understand the differences in affinity beween intra-species grafting of Castanea mollissima and C.henryi,inter-species grafting between C.mollissima and C.henryi,and to select wide affinity and rootstock combinations for practical application.[Method]Based on one-year-old grafted seedlings of four types of intra-species grafting and inter-species grafting of C.mollissima and C.henryi tetra species,the graft healing,growth traits,leaf phenotypes,and photosynthetic physiological indexes were investigated and measured,and cytological observations of graft healing were made using paraffin sections,and a comprehensive evaluation was carried out using statistical data.[Result](1)Among the four grafting types,the survival rate of intraspecific grafting of C.mollissima and C.henryi ranged from 35.71%to 91.33%with good healing of grafts,while the survival rate of interspecific grafting of C.mollissima and C.henryi ranged from 16.67%to 45.00%only with poor healing of grafts;(2)Seedling height,ground diameter,and leaf number were significantly(p<0.05)better in the C.mollissima or C.henryi intraspecific grafted combination than those of interspecific grafted combination;(3)There were differences in leaf phenotypic traits among different grafting types,with the leaf area and leaf thickness of intraspecific grafting combinations of C.mollissima being significantly greater than those of C.mollissima grafted with C.henryi.The petiole length of intraspecific grafting combinations of C.henryi was significantly larger than that of C.henryi i grafted with with C.mollissima;(4)Cytological observation revealed that the isolation layer between anvils and spikes disappeared in intraspecific grafting combinations of C.mollissima and C.henryi,and anvils and spikes were integrated,while an isolation layer appeared between anvils and spikes of interspecific grafting;(5)The comprehensive evaluation scores of each grafting combination showed that A4 was the best,followed by A7,A3,A2,A1,A8,A5,and A6.[Conclusion]There is a certain

关 键 词：板栗 锥栗 嫁接亲和性 生长性状 综合评价 

分 类 号：S727.3[农业科学—林木遗传育种] S664.2[农业科学—林学]