外源油菜素内酯及作用时间对赖草叶片光合特性的影响  

Effects of Exogenous Brassinolide and Duration of Action on the Photosynthetic Properties of Leymus secalinus Leaves

作　　者：廖明旺 秦士利 许辰雨 张倩[1] 王凯鑫 杜利霞[1] LIAO Ming-wang;QIN Shi-li;XU Chen-yu;ZHANG Qian;WANG Kai-xin;DU Li-xia(College of Grassland Science,Shanxi Agricultural University,Key Laboratory for Model Innovation in Forage Production Efficiency,Ministry of Agriculture and Rural Affairs,P.R.Taigu,Shanxi Province 030801,China)

机构地区：[1]山西农业大学草业学院,农业农村部饲草高校生产模式创新重点实验室,山西太谷030801

出　　处：《草地学报》2024年第12期3788-3796,共9页Acta Agrestia Sinica

基　　金：山西省重点研发计划(农业领域)(202102140601006);山西农业大学“十四五”生物育种工程项目(YZGC134);农业农村部饲草高校生产模式创新重点实验室;山西省重点实验室项目(202104010910017)共同资助。

摘　　要：为探究油菜素内酯(Brassinolide,BR)对赖草(Leymus secalinus)叶片光合能力的影响及作用时间,采用室内盆栽试验,叶面喷施不同浓度(0,0.05,0.1,0.15,0.2μmol·L^(-1))的BR,在7,14,21 d后分别测定光合相关指标。结果表明,BR处理可以显著提高赖草叶片的叶绿素含量、净光合速率、气孔导度、蒸腾速率、PSⅡ最大光化学量子产量、PSⅡ实际光量子产量、光化学淬灭系数、可溶性糖含量,显著降低了胞间CO_(2)浓度、非光化学淬灭系数。不同浓度BR影响赖草光合能力的作用时间不同,0.05μmol·L^(-1)BR处理的作用时间为7 d,0.1μmol·L^(-1)BR处理作用时间为14 d,喷施0.15和0.2μmol·L^(-1)BR处理下的持续时间为21 d。因此,在赖草实际生产中,通过叶面喷施0.15μmol·L^(-1)BR可以有效提升赖草的光合能力从而积累更多的光合产物,其作用周期在21 d左右。Here we conducted an indoor pot experiment to investigate the effects and duration of action of brassinolide(BR)on the photosynthetic capacity of Leymus secalinus leaves.In the experiment,different concentrations of BR(0,0.05,0.1,0.15,0.2μmol·L^(-1))were sprayed on the foliage,and the photosynthesis-related indexes were measured after 7,14 and 21 d,respectively.The results showed that BR treatments significantly increased the chlorophyll content,net photosynthetic rate,stomatal conductivity,transpiration rate,maximum photochemical quantum yield of PSII,actual photochemical quantum yield of PSII,photochemical quenching coefficient and soluble sugar in Leymus secalinus leaves,and significantly reduced Intercellular CO_(2) concentration,non-photochemical quenching coefficient.Different concentrations of BR affected the photosynthetic capacity of Leymus secalinus for different durations,the duration of the effect of 0.05μmol·L^(-1)BR treatment was 7 d,the duration of the effect of 0.1μmol·L^(-1)BR treatment was 14 d,and the duration of the effect of spraying 0.15 and 0.2μmol·L^(-1)BR treatments was 21 d.Therefore,in the actual production of Leymus secalinus,the photosynthetic capacity of Leymus secalinus can be effectively enhanced by foliar spraying of 0.15μmol·L^(-1)BR thereby accumulating more photosynthetic products,with an action cycle of about 21 d.

关 键 词：赖草 油菜素内酯 叶绿素 光合特性 荧光参数 

分 类 号：S543.9[农业科学—作物学]