CeFe nanofibrous carbon nanozyme integrated with smartphone for the point-of-care testing of norfloxacin in water  

作　　者：Yue Liu Taimei Cai Sen Chen Tao Wen Hailong Peng 

机构地区：[1]Key Laboratory of Natural Microbial Medicine Research of Jiangxi Province,School of Life Science,Jiangxi Science and Technology Normal University,Nanchang,330013,China [2]School of Chemistry and Chemical Engineering,Nanchang University,Nanchang,330031,China [3]College of Mechanical and Intelligent Manufacturing,Central South University of Forestry and Technology,Changsha,410004,China

出　　处：《Journal of Pharmaceutical Analysis》2024年第10期1505-1513,共9页药物分析学报（英文版）

基　　金：This work was financially supported by Natural Science Foundation of Jiangxi Province(Grant Nos.:20224ACB203016 and 20224BAB203022);Science and Technology Research Project of Jiangxi Provincial Department of Education(Grant No.:GJJ2201322);the National Natural Science Foundation of China(Grant Nos.:32060577 and 32360619);Natural Science Foundation for Distinguished Young Scholars of Hunan Province(Gtant No.:2023JJ10099).

摘　　要：The overuse of antibiotics has led to the severe contamination of water bodies,posing a considerable hazard to human health.Therefore,the development of an accurate and rapid point-of-care testing(POCT)platform for the quantitative detection of antibiotics is necessary.In this study,Cerium oxide(CeO_(2))and Ferrosoferric oxide(Fe_(3)O_(4))nanoparticles were simultaneously encapsulated into N-doped nanofibrous carbon microspheres to form of a novel nanozyme(CeFe-NCMzyme)with a porous structure,high surface area,and N-doped carbon material properties,leading to a considerable enhancement of the peroxidase(POD)-like activity compared with that of the CeO_(2)or Fe3O4 nanoparticles alone.The POD-like activity of CeFe-NCMzyme can be quenched using L-Cysteine(Cys)and subsequently restored by the addition of a quinolone antibiotic(norfloxacin,NOR).Therefore,CeFe-NCMzyme was used as a colorimetric sensor to detect NOR via an“On-Off”model of POD-like activity.The sensor possessed a wide linear range of 0.05–20.0μM(R^(2)=0.9910)with a detection limit of 35.70 nM.Furthermore,a smartphone-assisted POCT platform with CeFe-NCMzyme was fabricated for quantitative detection of NOR based on RGB analysis.With the use of the POCT platform,a linear range of 0.1–20.0μM and a detection limit of 54.10 nM were obtained.The spiked recoveries in the water samples were ranged from 97.73%to 102.01%,and the sensor exhibited good accuracy and acceptable reliability.This study provides a portable POCT platform for the on-site and quantitative monitoring of quinolone antibiotics in real samples,particularly in resource-constrained settings.

关 键 词：CeO_(2)nanoparticles Peroxidase-like activity Nanofibrous carbon microspheres Point-of-care testing NORFLOXACIN 

分 类 号：R446.1[医药卫生—诊断学]