续苓健骨方对骨质疏松模型大鼠骨密度的影响及作用机制  

The Effect and Mechanism of Shuling-jiangu Prescription on Bone Mineral Density in Osteoporosis Animal Models

作　　者：王政团 程赟 WANG Zheng-Tuan;CHENG Yun(Department of Orthopedics,Ezhou Egang Hospital,Ezhou 436000,China)

机构地区：[1]鄂州市鄂钢医院骨科,湖北鄂州436000

出　　处：《中国药物经济学》2024年第11期106-110,120,共6页China Journal of Pharmaceutical Economics

摘　　要：目的探讨续苓健骨方对骨质疏松大鼠骨密度的影响及作用机制。方法50只雌性SD大鼠根据随机数字表法分为假手术组、模型组、续苓健骨方干预组(低、中、高剂量),每组10只。模型组、干预组均采用双侧卵巢切除术建立骨质疏松模型。建模30 d后开始灌胃给药,其中低、中、高剂量组每天分别灌胃给予7.5 mg/kg、15 mg/kg、30 mg/kg的续苓健骨方,假手术组、模型组给予等体积的0.9%氯化钠注射液。连续给药12周后,检测骨密度、骨微细结构,测试骨生物力学,采用酶联免疫吸附试验法检测血清骨保护蛋白、抗酒石酸酸性磷酸酶水平,采用Real-time PCR技术检测p38丝裂原活化蛋白激酶信号通路中相关m RNA表达水平。结果5组大鼠骨最大载荷、三点弯曲应力以及骨密度差异显著,其中假手术组显著高于其他4组,模型组显著低于其他4组,随着给药剂量的增加,干预组最大载荷、三点弯曲应力以及骨密度均呈明显升高趋势,差异有统计学意义(P<0.05)。治疗12周后干预组骨小梁数量、骨小梁厚度、骨表面积与体积比明显高于模型组,且随着剂量增加,各值均呈明显升高趋势,差异有统计学意义(P<0.05);干预组骨小梁间隙显著低于模型组,且随着剂量增加呈明显降低趋势,差异有统计学意义(P<0.05)。治疗12后模型组血清抗酒石酸酸性磷酸酶水平明显增加,且高于假手术组,而干预组明显降低,随着给药剂量的增加,血清抗酒石酸酸性磷酸酶水平呈明显降低趋势,差异有统计学意义(P<0.05);模型组血清骨保护蛋白水平明显降低,而干预组明显增加,随着给药剂量的增加,血清骨保护蛋白水平呈明显升高趋势,差异有统计学意义(P<0.05)。模型组血清MKK4、P38α、P38γ、基质金属蛋白酶-3、基质金属蛋白酶-13的m RNA表达水平明显高于假手术组、干预组,干预组随着给药剂量的增加,血清MKK4、P38α、P38γ、基质金属Objective To explore the effect and mechanism of Xulingjiangu formula on bone mineral density in osteoporosis rats.Methods Fifty female SD rats were divided into sham operation group,model group and Xulingjiangu prescription intervention group(low,medium and high dose)according to random number table method,with 10 rats in each group.The osteoporosis model was established by bilateral oophorectomy in both the model group and the intervention group.After 30 days of modeling,the administration was started by intragastric administration,in which the low,medium and high dose groups were given 7.5 mg/kg,15 mg/kg and 30 mg/kg Xulingjiangu prescription by intragastric administration,respectively,and the sham operation group and model group were given equal volume 0.9%sodium chloride injection.After 12 weeks of continuous administration,bone mineral density,bone microstructure and bone biomechanics were detected.Serum levels of bone protective protein and tartrate-resistant acid phosphatase were detected by enzyme-linked immunosorbent assay(ELISA).mRNA expression levels in p38 mitogen-activated protein kinase signaling pathway were detected by Real-time PCR.Results The maximum bone load,three-point bending stress and bone mineral density of rats in the 5 groups had significant differences,among which the sham operation group was significantly higher than the other 4 groups,and the model group was significantly lower than the other 4 groups.With the increase of administration dose,the maximum load,three-point bending stress and bone mineral density of rats in the intervention group showed a significant increase trend,and the difference was statistically significant(P<0.05).After 12 weeks of treatment,the number of bone trabeculae,the thickness of bone trabeculae and the ratio of bone surface area to volume in the intervention group were significantly higher than those in the model group,and with the increase of dose,all values showed a significant increasing trend(P<0.05).The bone trabecular space in the intervention grou

关 键 词：P38丝裂原活化蛋白激酶 骨质疏松 骨密度 骨微细结构 骨生物力学 

分 类 号：R681.1[医药卫生—骨科学] R289.5[医药卫生—外科学]