不同浓度胃蛋白酶气溶胶对慢性阻塞性肺疾病大鼠的建模及作用机制探究  

Modeling and Mechanism of Action of Gastric Protease Aerosols with Different Concentrations in Rats with Chronic Obstructive Pulmonary Disease

作　　者：许腾[1] 刘红春[1] 崔彦杰[1] 赵炳尧 郝立君[1] XU Teng;LIU Hong-chun;CUI Yan-jie;ZHAO Bing-yao;HAO Li-jun(Department of Laboratory Medicine,People's Hospital of Xinjiang Uygur Autonomous Region,Urumqi,Xinjiang,830001,China)

机构地区：[1]新疆维吾尔自治区人民医院检验科,新疆乌鲁木齐830001

出　　处：《现代生物医学进展》2024年第21期4054-4056,4122,共4页Progress in Modern Biomedicine

基　　金：新疆维吾尔自治区人民医院院级科研项目(20230205);新疆维吾尔自治区自然科学基金项目(2021D01C152)。

摘　　要：目的:探讨不同浓度胃蛋白酶气溶胶对慢性阻塞性肺疾病大鼠的建模及作用机制。方法:48只雄性SD大鼠分组,各16只。低、高浓度组分别给与75 ng/mL、150 ng/mL胃蛋白酶气溶胶,正常对照组给予过滤空气,对比3组相关指标。结果:造模结束后体重为正常对照组>低浓度组>高浓度组,L<0.05。3组大鼠的肺功能指标、血气分析指标、血常规、炎性因子、以及肺组织蛋白表达有统计学意义,P均<0.05。HE染色后,低浓度组大鼠肺组织气道周围出现炎性细胞浸润,支气管壁增厚,肺泡间隔增宽,邻近的肺泡腔、肺泡组织出现代偿性的扩张;高浓度组大鼠肺组织与低浓度组相似。结论:150 ng/mL胃蛋白酶气溶胶建立的模型更符合慢性阻塞性肺疾病临床、病理特点,可能与炎症浸润及氧化应激有关。Objective:To investigate the modeling and mechanism of action of different concentrations of gastric protease aerosols on rats with chronic obstructive pulmonary disease.Methods:48 male SD rats were divided into groups of 16 each.The low and high concentration groups were given 75 ng/mL and 150 ng/mL gastric protease aerosols,respectively,while the normal control group was given filtered air.The relevant indicators of the three groups were compared.Results:After modeling,the body weight of the three groups of rats was normal control group>low concentration group>high concentration group,with P<0.05.The lung function indicators,blood gas analysis indicators,blood routine,inflammatory factors,and lung tissue protein expression of the three groups of rats had statistical significance,with P<0.05.After HE staining,inflammatory cell infiltration appeared around the airway in the lung tissue of rats in the low concentration group,with thickening of the bronchial wall,widening of the alveolar septum,and compensatory dilation of adjacent alveolar cavities and alveolar tissues;The lung tissue of rats in the high concentration group was similar to that in the low concentration group.Conclusion:The model established by 150 ng/mL gastric protease aerosol is more in line with the clinical and pathological characteristics of chronic obstructive pulmonary disease,and may be related to inflammatory infiltration and oxidative stress.

关 键 词：慢性阻塞性肺疾病 动物模型 胃蛋白酶气溶胶 

分 类 号：R563[医药卫生—呼吸系统]