Dynamics of Haptophytes(Eukaryota,Hacrobia)Revealed via Short-Term Sampling in the South China Sea  

作　　者：ZHANG Haoyue LI Ran GU Bowei KONG Hejun XU Jie ZHANG Rui LI Xiaolin JIAO Nianzhi XU Dapeng 

机构地区：[1]College of Ocean and Earth Sciences,State Key Laboratory of Marine Environmental Science,Xiamen University,Xiamen 361102,China [2]Institute of Marine Microbes and Ecospheres,Fujian Key Laboratory of Marine Carbon Sequestration,Xiamen University,Xiamen 361102,China [3]Centre for Regional Oceans,Department of Ocean Science and Technology,Faculty of Science and Technology,University of Macao,Macao 999078,China [4]Institute for Advanced Study,Shenzhen University,Shenzhen 518060,China

出　　处：《Journal of Ocean University of China》2024年第6期1677-1688,共12页中国海洋大学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.42276095,42188102,42141003,and U20A20103);the Open Innovation Fund for Undergraduate Students of Xiamen University,and Ocean Negative Carbon Emissions(ONCE)program。

摘　　要：Haptophytes(Eukaryota,Hacrobia)play a crucial role in the energy budget and element cycling of diverse aquatic ecosystems due to their ability to engage in both phototrophic and mixotrophic nutritional modes.Nevertheless,there is a significant lack of knowledge regarding the short-term variations,such as diel dynamics,of their ecological features.During a short time frame in the summer of 2018,samples were collected from three distinct water layers in the South China Sea,including surface water,the deep chlorophyll maximum(DCM)layer,and 200 m depth.Fluorescence in situ hybridization coupled with tyramide signal amplification was used to quantify haptophyte cell abundance.Most haptophyte communities in all three water layers were composed of cells 2-5μm in size,while the proportion of cells<2μm increased with water depth.High-throughput sequencing of the V4 hypervariable regions of the SSU rRNA revealed that Chrysochromulina and Phaeocystis predominated the community,and the former was more abundant in the surface water and 200 m depth and the latter was more abundant in the DCM layer.Higher abundance of small cells(<2μm and 2-5μm)during the night was found compared to the day time,whereas large cells(5-10μm and 10-20μm)were more prevalent during the day time.The results of correlation analyses showed that haptophyte abundance was possibly impacted by both environmental biotic(heterotrophic nanoflagellates,heterotrophic bacteria,and viruses)and abiotic(temperature,salinity,and nutrients)factors.

关 键 词：eukaryotic phytoplankton FISH-TSA high-throughput sequencing microbial eukaryotes size fraction 

分 类 号：Q948.8[生物学—植物学]