分析酶联免疫吸附法及RT-PCR法检验血液标本内乙肝病毒的价值  

Value Analysis of Enzyme-Linked Immunosorbent Assay and RT-PCR In Detecting Hepatitis B Virus of Blood Samples

作　　者：冯慧雅 黄树通 卜丹霞[1] 陈彩仁 赖石凤 FENG Huiya;HUANG Shutong;BU Danxia;CHEN Cairen;LAI Shifeng(Laboratory Department,Zhanjiang Central People's Hospital,Zhanjiang,Guangdong 524000)

机构地区：[1]湛江中心人民医院检验科,广东湛江524000

出　　处：《智慧健康》2024年第27期5-7,共3页Smart Healthcare

摘　　要：目的探讨酶联免疫吸附法及RT-PCR法检验血液标本内乙肝病毒的价值。方法将2022年1月—2023年10月在本院治疗的78例疑似乙肝患者作为研究对象,根据检测方法不同分为A组和B组,每组39例。两组使用酶联免疫吸附法(ELISA)、实时荧光定量PCR(RT-PCR)检测乙肝病毒(HBV)血清标志物、HBV-DNA含量。其中,A组采用单纯ELISA检验,B组采用单纯RT-PCR检验。以临床最终诊断结果作为金标准,分析两组检测结果,对比诊断效能。结果A组中,HbeAg阳性率39.74%(31/78),HBsAg阳性率58.97%(46/78),HbcAb阳性率51.28%(40/78),HbeAb阳性率56.41%(44/78),HbsAb阳性率61.54%(48/78),大三阳的阳性率39.74%(31/78),小三阳的阳性率51.28%(40/78);B组中,HBV-DNA阳性率80.77%(63/78);A组对HBV的诊断敏感度、准确度、阴性预测值均低于B组(P<0.05),但两组特异度、阳性预测值相当(P>0.05)。结论RT-PCR法检验血液标本内HBV的价值更高,对HBV的敏感度及准确度高,整体诊断效能更高。Objective To explore the value of enzyme-linked immunosorbent assay(ELISA)and RT-PCR in detecting hepatitis B virus of blood samples.Methods The paper chose 78 patients with suspected hepatitis B in our hospital from January 2022 to October 2023 as research subjects,and detected Hepatitis B virus(HBV)serum markers and HBV-DNA contents with enzyme linked immunosorbent assay(ELISA)and real-time fluorescent quantitative PCR(RT-PCR).Those with simple ELISA test was included in the group A,and those with simple RT-PCR test in group B.With gold standard of final clinical diagnosis result,test results were analyzed,diagnostic efficacy was compared between two groups.Results In group A,the positive rate of HbeAg was 39.74%(31/78),that of HBsAg was 58.97%(46/78),that of HbcAb was 51.28%(40/78),that of HbeAb was 56.41%(44/78),that of HbsAb was 61.54%(48/78),that of HBsAb was 39.74%(31/78),that of HBsAg,HBeAg and HBcAb was 39.74%(31/78),that of HBsAg,HBeAb and HBcAb was 51.28%(40/78).In group B,the positivity rate of the HBV-DNA was 80.77%(63/78).The diagnostic sensitivity,accuracy,and negative predictive value of group A for HBV were lower than group B(P<0.05),but the specificity and positive predictive value between two groups was comparable(P>0.05).Conclusion The RT-PCR method has higher value,sensitivity,and accuracy in detecting HBV in blood samples,which has higher overall diagnostic effect.

关 键 词：血液标本 乙肝病毒 酶联免疫吸附法 实时荧光定量PCR 诊断效果 

分 类 号：R512.62[医药卫生—内科学] R446.6[医药卫生—临床医学]