接骨木新品种盐丹对大鼠骨折愈合的促进作用研究  

The promoting effect of a new variety of Sambucus williamsii Hance Yandan on fracture healing in rats

作　　者：刘洋 郭庆梅[1] 姚俊修[2] 袁俊鹏 王云达 LIU Yang;GUO Qingmei;YAO Junxiu;YUAN Junpeng;WANG Yunda(College of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan 250355,China;Shandong Academy of Forestry/Shandong Provincial Key Laboratory of Genetic Breeding of Ecological Forest and Grass,Jinan 250014,China)

机构地区：[1]山东中医药大学药学院,济南250355 [2]山东省林业科学研究院/山东省生态林草遗传育种重点实验室,济南250014

出　　处：《中国药房》2024年第24期3004-3009,共6页China Pharmacy

基　　金：山东省重点研发计划(No.2020LZGC090502)。

摘　　要：目的研究接骨木新品种盐丹对大鼠骨折愈合的影响。方法将SD大鼠随机分为假手术组、模型组、中华跌打丸组(0.54 g/kg)、野生接骨木组(5.4g/kg,以生药量计)和盐丹高、中、低剂量组(10.8、5.4、2.7g/kg,以生药量计),每组12只。除假手术组外,其余各组大鼠制备股骨骨折模型。于术后第2天起,各组大鼠灌胃相应药物和蒸馏水,每日1次,连续8周。于给药2、4、8周后,检测大鼠血清中钙、磷、碱性磷酸酶(ALP)和骨钙素(OCN)水平,利用Micro-CT扫描评价骨折股骨形态和骨微结构参数[骨密度(BMD)、骨体积/组织体积(BV/TV)、骨小梁数量(Tb.N)、骨小梁分离度(Tb.Sp)、骨小梁厚度(Tb.Th)],采用苏木精-伊红染色观察骨组织骨折处的形态变化。结果与模型组比较,盐丹高剂量组大鼠给药2、4、8周后血清中钙水平(给药4周除外)均显著降低,磷、ALP、OCN水平均显著升高(P<0.05);骨折部位可见骨性骨痂形成、骨折断端连接、骨折线逐渐模糊或消失、骨髓腔开通等骨修复过程,骨组织骨折处可见大量肉芽组织、成纤维细胞、软骨细胞、新生骨小梁以及新生骨板等;给药8周后的BMD、BV/TV、Tb.N、Tb.Th均显著升高,Tb.Sp显著降低(P<0.05)。野生接骨木组上述大部分指标变化不明显。结论盐丹具有促进大鼠骨折愈合的作用,且作用优于野生接骨木。OBJECTIVE To investigate the effect of a new variety of Sambucus williamsii Hance Yandan on the healing in rats with fractures.METHODS SD rats were randomly allocated to sham surgery group,model group,Zhonghua dieda pill group(0.54 g/kg),wild S.williamsii group(5.4 g/kg,using raw drug dosage),and high-,medium-,and low-dose groups of Yandan(10.8,5.4,2.7 g/kg,using raw drug dosage),with 12 rats in each group.Except for the sham surgery group,the remaining groups were prepared with a femoral fracture model.Starting from the second day after surgery,each group was intubated with the corresponding drugs and distilled water once daily for 8 consecutive weeks.At 2,4,and 8 weeks after administration,the levels of calcium,phosphorus,alkaline phosphatase(ALP),and osteocalcin(OCN)in rat serum were detected.Micro-CT scanning was used to evaluate the morphology and bone microstructural parameters of the fractured femur[bone mineral density(BMD),bone volume/tissue volume(BV/TV),trabecular number(Tb.N),trabecular separation(Tb.Sp),trabecular thickness(Tb.Th)],and hematoxylin-eosin staining was adopted to observe the morphological changes at the bone fracture site.RESULTS Compared with the model group,the levels of calcium in rat serum were significantly decreased(except at 4 weeks after administration),and the levels of phosphorus,ALP,and OCN were significantly increased(P<0.05)at 2,4,and 8 weeks after administration in the high-dose group of Yandan;bone callus formation,connection of fracture ends,gradual blurring or disappearance of the fracture line,and opening of the marrow cavity were observed in the bone repair process,and a large amount of granulation tissue,fibroblasts,chondrocytes,new trabeculae,and new bone plates were visible at the fracture site of bone tissue;after 8 weeks of administration,BMD,BV/TV,Tb.N,and Tb.Th were significantly increased,and Tb.Sp was significantly decreased(P<0.05).Most of the above indicators in the wild S.williamsii Hance group showed no significant changes.CONCLUSIONS Yandan has the effec

关 键 词：接骨木 盐丹 骨折 骨折愈合 钙磷平衡 

分 类 号：R965[医药卫生—药理学]