灯盏乙素对脑缺血大鼠小胶质细胞SIRT1/NF-κB通路的影响  

The effects of scutellarin on SIRT1/NF-κB signaling pathway in microgial of cerebral ischemic rat

作　　者：徐冬垚 段兆达 杨毓甲 彭应琪 吴雨珂 杨力 吴春云 XU Dongyao;DUAN Zhaoda;YANG Yujia;PENG Yingqi;WU Yuke;YANG Li;WU Chunyun(Department of Human Anatomy and Histology&Embryology,Faculty of Basic Medical Sciences,Kunming Medical University,Kunming Medical University,Kunming 650500,China;First School of Clinical Medicine,Kunming Medical University,Kunming 650500,China)

机构地区：[1]昆明医科大学基础医学院人体解剖与组织胚胎学系,昆明650500 [2]昆明医科大学第一临床医学院,昆明650500

出　　处：《神经解剖学杂志》2024年第5期581-587,共7页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(81960223);云南省科技厅联合专项(202301AY070001-163);云南省教育厅科学研究基金研究生项目(2024Y206)。

摘　　要：目的:探究灯盏乙素干预大鼠脑缺血小胶质细胞(MG)中沉默信息调节因子2相关酶1/核因子κB(SIRT1/NF-κB)信号通路的表达变化。方法:雄性SD大鼠随机分为假手术组(Sham组)、大脑中动脉闭塞组(MCAO组)、MCAO+灯盏乙素干预组(MCAO+S组)。Western Blot和免疫荧光染色检测大鼠脑缺血皮质区SIRT1、NF-κB(p65)的表达变化和NF-κB磷酸化水平(p-p65)。结果:Western Blot和免疫荧光染色结果显示灯盏乙素干预显著促进大鼠脑缺血后缺血皮质区SIRT1的表达,显著降低NF-κB的磷酸化水平(P<0.05)。结论:灯盏乙素对脑缺血大鼠小胶质细胞SIRT1/NF-κB信号通路具有调控作用。Objective:Investigating the effect of scutellarin on the expression of silent information regulator 1/nuclear factorκB(SIRT1/NF-κB)signaling pathway in microglia of cerebral ischemic rat.Methods:Male SD rats were devided into sham group,middle cerebral artery occlusion group(MCAO group),MCAO+scutellarin treatment group(MCAO+S group)randomly.Western Blot and immunofluorescence were used to detect the expression of SIRT1 and NF-κB(p65)and phosphorylation of NF-κB(p-p65)of ischemic cortex of rats.Results:Results of Western Blot and immunofluorescence showed that scutellarin pretreatment significantly increased the expression of SIRT1 and reduced phosphorylation of NF-κB p65(P<0.05).Conclusion:Scutellarin could regulate SIRT1/NF-κB signaling pathway in microglia of cerebral ischemic rat.

关 键 词：脑缺血 灯盏乙素 大脑中动脉闭塞 沉默信息调节因子2相关酶1/核因子κB 小胶质细胞 

分 类 号：R285.5[医药卫生—中药学]