pH对EAD中关键酶活性和产甲烷代谢通量的影响作用  

The Effect of pH on the Activity of Key Enzymes and Methane Production Flux in EAD

作　　者：付伟涛 刘洪周 王楠 刘海波 李建昌[1] FU Weitao;LIU Hongzhou;WANG Nan;LIU Haibo;LI Jianchang(School of Energy and Environmental Sciences,Yunnan Normal University,Kunming 650500,China)

机构地区：[1]云南师范大学能源与环境科学学院,云南昆明650500

出　　处：《云南师范大学学报（自然科学版）》2024年第6期15-20,共6页Journal of Yunnan Normal University:Natural Sciences Edition

基　　金：国家自然科学基金资助项目(21968038).

摘　　要：为探究pH对电化学厌氧消化(EAD)系统中关键酶活性和产甲烷代谢通量的影响作用,通过代谢通量分析以及冗余分析研究了pH调节前后EAD关键产物(CH_(4)、CO_(2)和H2等)和酶活性的变化.结果表明:随着pH的改变,会直接影响与产酸相关的酶,其中PTA、AK、PTB、BK、DH、ADH和LDH酶活性均高于对照组,这是由于调节pH,尤其是降低pH,会破坏EAD体系的稳定状态,挥发性脂肪酸大量积累,而产甲烷通量中关键性酶活性较低,因此导致产甲烷通量最小而pH为7.5扰动时则会表现出最佳产甲烷代谢通量.To investigate the effect of pH on the activity of key enzymes and methane production metabolic flux in electrochemical anaerobic digestion(EAD)systems,changes in key products(CH_(4),CO_(2),and H 2,etc.)and enzyme activity of EAD before and after pH adjustment were studied through metabolic flux analysis and redundancy analysis.The results showed that enzymes related to acid production were directly affected when pH changed.Among them,PTA,AK,PTB,BK,DH,ADH,and LDH enzyme activities were higher than those in the control group.This is because adjusting pH,especially lowering pH,can disrupt the stable state of the EAD system,leading to a large accumulation of volatile fatty acids.The key enzyme activity in methane production flux is relatively low,resulting in the minimum methane production flux.When pH is disturbed at 7.5,the optimal methane production metabolic flux is exhibited.

关 键 词：电化学厌氧消化 产甲烷代谢通量 pH扰动 酶活性 

分 类 号：X705[环境科学与工程—环境工程]