重金属铜对土壤磷酸酶活性的影响及其动力学特征  

Effects of Copper on Soil Phosphatase Activity and Its Kinetic Characteristics

作　　者：范梦林 樊芳玲 石炳春 唐振亚 姚昊翊 FAN Menglin;FAN Fangling;SHI Bingchun;TANG Zhenya;YAO Haoyi(School of Energy and Environment Science,Yunnan Normal University,Kunming 650500,China;Yunnan Key Laboratory of Rural Energy Engineering,Kunming 650500,China;Faculty of Modern Agricultural Engineering,Kunming University of Science and Technology,Kunming 650500,China)

机构地区：[1]云南师范大学能源与环境科学学院,云南昆明650500 [2]云南省农村能源工程重点实验室,云南昆明650500 [3]昆明理工大学现代农业工程学院,云南昆明650500

出　　处：《云南师范大学学报（自然科学版）》2024年第6期66-70,共5页Journal of Yunnan Normal University:Natural Sciences Edition

基　　金：国家自然科学基金资助项目(42107256);云南省基础研究计划资助项目(202201AT070036,202201AU070147,202301AT070460);云南省大学生创新创业计划资助项目(S202310681103X)。

摘　　要：为了获得不同浓度的Cu对土壤磷酸酶活性的影响及其动力学特征,研究了Cu与土壤磷酸酶活性和酶促反应参数之间的关系.结果表明:土壤磷酸酶活性随着底物浓度的增加而增加;在相同底物浓度下,土壤磷酸酶活性随Cu浓度的增加而降低;底物浓度、铜浓度相同时磷酸酶活性存在差异,这与土壤磷酸酶的酸度和碱度有关;铜与土壤磷酸酶的最大反应速度V_(max)呈现极显著相关,表明可以用V_(max)来表征土壤受重金属Cu的污染水平.In order to obtain the effects of different concentrations of copper on soil phosphatase activity and its kinetic characteristics,the relationship between copper and soil phosphatase activity and enzymatic reaction parameters was studied.The results showed that soil phosphatase activity increased with the increase of substrate concentration.At the same substrate concentration,soil phosphatase activity decreased with the increase of copper concentration.When the substrate concentration and copper concentration are the same,there are differences in phosphatase activity,which is related to the acidity and alkalinity of soil phosphatase.There is a very significant correlation between copper and the maximum reaction rate V_(max) of soil phosphatase,indicating that V_(max) can be used to characterize the soil pollution level with heavy metal Cu.

关 键 词：重金属 铜 土壤磷酸酶 酶动力学参数 土壤污染 

分 类 号：S154.2[农业科学—土壤学]