肺炎支原体肺炎患儿血清lncRNA GAS5、TIMP3水平变化及临床意义  

Changes in serum lncRNA GAS5 and TIMP3 levels in children with mycoplasma pneumoniae pneumonia and their clinical significance

作　　者：韩彩云 马娇 李晓慧 HAN Caiyun;MA Jiao;LI Xiaohui(Department of Pediatrics,Yulin Xingyuan Hospital,Yulin,Shaanxi 719000,China;Department of Pediatrics,Shenmu Hospital of Integrated Traditional Chinese and Western Medicine,Shenmu,Shaanxi 719399,China)

机构地区：[1]榆林市星元医院儿科,陕西榆林719000 [2]陕西省神木市中西医结合医院儿科,陕西神木719399

出　　处：《国际检验医学杂志》2024年第24期2983-2988,共6页International Journal of Laboratory Medicine

基　　金：陕西省科技计划项目(2020SF-138)。

摘　　要：目的 探讨肺炎支原体肺炎(MPP)患儿血清长链非编码核糖核酸(lncRNA)生长抑制特异性基因5(GAS5)、组织金属蛋白酶抑制剂(TIMP)3水平变化及临床意义。方法 选取2021年1月至2023年10月榆林市星元医院儿科收治的MPP患儿150例作为MPP组,根据病情严重程度分为重症组(98例)和轻症组(52例),根据28 d预后情况分为不良预后组(31例)和良好预后组(119例),另选取同期60例健康儿童作为对照组。采用实时荧光定量聚合酶链反应与酶联免疫吸附试验分别检测血清lncRNA GAS5及TIMP3水平。通过Pearson相关性分析血清lncRNA GAS5、TIMP3水平在MPP患儿中的相关性,采用多因素Logistic回归分析影响MPP患儿不良预后的因素,受试者工作特征曲线分析血清lncRNA GAS5、TIMP3水平对MPP患儿不良预后的预测价值。结果 重症组血清lncRNA GAS5、TIMP3水平低于轻症组和对照组(P<0.05),轻症组血清lncRNA GAS5、TIMP3水平低于对照组(P<0.05)。MPP患儿血清lncRNA GAS5与TIMP3水平呈正相关(r=0.633,P<0.001)。150例MPP患儿28 d不良预后发生率为20.67%(31/150)。不良预后组重症比例、峰值体温、降钙素原、C反应蛋白水平高于良好预后组(P<0.05),住院时间长于良好预后组(P<0.05),lncRNA GAS5、TIMP3水平低于良好预后组(P<0.05)。重症、降钙素原水平升高为MPP患儿不良预后的独立危险因素(P<0.05),lncRNA GAS5和TIMP3水平升高为其独立保护因素(P<0.05)。血清lncRNA GAS5联合TIMP3水平预测MPP患儿不良预后的曲线下面积为0.878,大于血清lncRNA GAS5、TIMP3水平单独预测的0.780、0.778(P<0.05)。结论 MPP患儿血清lncRNA GAS5、TIMP3水平降低与病情加重及不良预后密切相关,血清lncRNA GAS5联合TIMP3对MPP患儿不良预后有较高的预测价值。Objective To investigate the changes in the levels of serum long noncoding ribonucleic acid(lncRNA)growth arrest specific 5(GAS5)and tissue inhibitor of matrix metalloproteinases(TIMP)3 and the clinical significance in children with mycoplasma pneumoniae pneumonia(MPP).Methods A total of 150 children with MPP admitted to Department of Pediatrics of Yulin Xingyuan Hospital from January 2021 to October 2023 were selected as MPP group and were divided into a severe group(98 cases)and a mild group(52 cases)according to the severity of disease,and were divided into a poor prognosis group(31 cases)and a good prognosis group(119 cases)according to the 28 d prognosis.Another 60 healthy children during the same period were selected as the control group.Serum lncRNA GAS5 and TIMP3 levels were measured by real-time fluorescence quantitative polymerase chain reaction and enzyme-linked immunosorbent assay respectively.Pearson correlation was used to analyze the correlation of serum lncRNA GAS5 and TIMP3 levels in children with MPP,multivariate Logistic regression was used to analyze the factors affecting the poor prognosis in children with MPP,and receiver operating characteristic curve was used to analyze the predictive value of serum lncRNA GAS5 and TIMP3 levels for poor prognosis in children with MPP.Results Serum lncRNA GAS5 and TIMP3 levels in the severe group were lower than those in the mild group and the control group(P<0.05),and serum lncRNA GAS5 and TIMP3 levels in the mild group were lower than those in the control group(P<0.05).Serum lncRNA GAS5 and TIMP3 levels were positively correlated in children with MPP(r=0.633,P<0.001).The incidence of 28 d poor prognosis in 150 children with MPP was 20.67%(31/150).The proportion of severe cases,peak body temperature,procalcitonin,and C-reactive protein levels in the poor prognosis group were higher than those in the good prognosis group(P<0.05),the length of hospital stay was longer than that in the good prognosis group(P<0.05),and the levels of lncRNA GAS5 and TIMP3 were

关 键 词：肺炎支原体肺炎 长链非编码核糖核酸生长抑制特异性基因5 组织金属蛋白酶抑制剂3 病情严重程度 预后 

分 类 号：R563[医药卫生—呼吸系统]